Gas Chromatography/Mass Spectrometry
Springer Berlin (Verlag)
978-3-642-82614-6 (ISBN)
The Use of Combined Gas Chromatography-Mass Spectrometry in the Analysis of Plant Growth Substances.- 1 Introduction.- 2 Identification by GC-MS.- 3 Gibberellins.- 4 Abscisic Acid and Related Compounds.- 5 Brassinosteroids.- References.- Applications of Mass Spectrometry for the Examination of Pectic Polysaccharides.- 1 Introduction.- 2 Structural Analysis of Pectic Polysaccharides.- 3 Determination of the Nature of the Glycosidic Linkages.- 4 Separation and Identification of Partially Methylated Alditol Acetates.- 5 Extensions of Methylation Analysis.- 6 Sequencing of Sugar Residues in Pectins.- 7 Sequencing of Pectic Polysaccharides by Partial Depolymerisation of Permethylated Derivatives.- 8 Experimental.- References.- GC-MS Methods for Cyclic Nucleotides in Higher Plants and for Free High Unsaturated Fatty Acids in Oils.- 1 Introduction.- 2 Methods of GC-MS.- 3 Adenosine-3?:5?-Monophosphate (cAMP) in Maize Seedlings (Zea mays).- 4 An Isotope Dilution GC-MS Spectrometric Assay for cAMP in Cultured Tobacco Tissue.- 5 Stability of Cyclic Purine Nucleotides in the Presence of Hydrochloric Acid During Extraction.- 6 Guanosine-3?:5?-Monophosphate (cGMP) in Maize Seedlings (Zea mays).- 7 GC-Separation of Synthetic cAMP and cGMP in a Mixture.- 8 Cyclic Pyrimidine Nucleotides in Plants?.- 9 Free High Unsaturated Fatty Acids in Oils.- 10 Conclusions.- References.- GC-MS Methods for Lower Plant Glycolipid Fatty Acids.- 1 Introduction.- 2 Extraction of the Plant Material.- 3 Separation of Glycolipids from the Total Lipid Extract.- 4 Isolation of the Glycolipids.- 5 Derivatization of the Glycolipid Fatty Acids for GC-MS.- 6 GLC Instrumentation for Fatty Acid Analysis.- 7 Column Selection for Fatty Acid GLC Analysis.- 8 Interpretation of GC Data and Calculation ofResults.- 9 Fatty Acid Ester Structure Determination by GC-MS.- 10 Abbreviations.- References.- Analysis of Phospholipid Molecular Species by Gas Chromatography and Coupled Gas Chromatography-Mass Spectrometry.- 1 Introduction.- 2 Lipid Preparation.- 3 Formation of Derivatives for GC or GC-MS.- 4 Gas Chromatography.- 5 Mass Spectrometry.- 6 Determination of Positional Distribution of Acyl Chains Using Phospholipase A2.- 7 Conclusion.- References.- GC-MS of Plant Sterol Analysis.- 1 Introduction.- 2 Development of GC-MS Plant Sterol Analysis.- 3 Operations Before GC-MS Sterol Analysis.- 4 Characterization of Sterols.- 5 Conclusion.- References.- GC-MS Methods for Terpenoids.- 1 Introduction.- 2 Isolation Methods.- 3 Prefractionation and Ancillary Reactions.- 4 Gas Chromatography.- 5 Retention Data.- 6 Mass Spectrometry.- References.- GC-MS of Auxins.- 1 Introduction.- 2 The Compounds Involved.- 3 Reference Compounds.- 4 Extraction.- 5 Purification.- 6 Columns for GC.- 7 Injection Techniques.- 8 Derivatisation.- 9 Interface Between GC and MS.- 10 Mass Spectrometer.- 11 Data Systems.- 12 Ionization.- 13 GC-MS Strategy for Auxin Analysis.- 14 Quantification.- 15 The Internal Standard.- 16 Experimental Procedure.- 17 Conclusions.- References.- GC-MS Methods for the Quantitative Determination and Structural Characterization of Esters of Indole-3-Acetic Acid and myo-Inositol.- 1 Introduction.- 2 Quantitative Analysis and Identification of the IAA-Inositols.- 2.2.1 A Quantitative Estimation of IAA-Inositol Using [3H]-IAA-myo-Inositol as an Internal Standard.- 3 Qualitative Analysis of IAA-Inositols.- 4 Conclusions.- 5 Abbreviations.- References.- GC-MS Methods for Cytokinins and Metabolites.- 1 Introduction.- 2 Gas Chromatography (GC).- 3 Mass Spectrometry.- 4 Applications ofMass Spectrometry in Cytokinin Analysis.- 5 General Remarks and Conclusion.- References.- GC-MS Method for Volatile Flavor Components of Foods.- 1 Introduction.- 2 GC-MS Methods.- 3 Volatile Flavor Components.- References.- GC-MS Methods for Tobacco Constituents.- 1 Introduction.- 2 Cembranoids and Their Degraded Compounds.- 3 Labdanoids and Their Degraded Compounds.- 4 Carotenoid-Degraded Compounds.- 5 Sesquiterpenoids.- 6 Terpenoid Glycosides.- 7 Linked Scanning.- References.
Erscheint lt. Verlag | 22.12.2011 |
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Reihe/Serie | Molecular Methods of Plant Analysis |
Co-Autor | R.S. Bandurski, G. Combaut, A. Ehmann, P. Hedden, B. Janistyn, H. Kameoka, H. Kodama, D.V. Lynch, J.K. Macleod, H. Nyberg, L.M.S. Palni, L. Rivier, R.R. Selvendran, B.J.H. Stevens, S.A.B. Tay, G.A. Jr. Thompson, L. Witte |
Zusatzinfo | XVI, 304 p. |
Verlagsort | Berlin |
Sprache | englisch |
Maße | 170 x 244 mm |
Gewicht | 560 g |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Schlagworte | Cells • Depolymerisation • Lipid • Nucleotide • Polysaccharid • Purine • Pyrimidine • Seedlings • tissue |
ISBN-10 | 3-642-82614-8 / 3642826148 |
ISBN-13 | 978-3-642-82614-6 / 9783642826146 |
Zustand | Neuware |
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