Protoplast Fusion

Genetic Engineering in Higher Plants

Y. Y. Gleba, K. M. Sytnik (Autoren)

Buch | Softcover

X, 222 Seiten

2011 | 1984
Springer Berlin (Verlag)
978-3-642-82247-6 (ISBN)

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Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.

1 Introduction.- 2 Techniques of Parasexual Hybridization.- 2.1 Culture Techniques.- 2.2 Isolated Protoplast Stage and Plant Cell Engineering.- 2.3 Problems of Genetic Variability.- 3 Protoplast Fusion and Parasexual Hybridization of Higher Plants.- 3.1 Perspectives.- 3.2 Methods of Screening for Parasexual Hybrids.- 3.3 Frequency of Formation of Parasexual Hybrids.- 3.4 Protoplast Fusion and the Bypassing of Sexual Barriers.- 3.5 Analysis of the Parasexual Hybridization Process and the Genetic Composition of Plant Forms Arising from Protoplast Fusion.- 4 Transmission Genetics of Parasexual Hybridization in Closely Related Crosses.- 4.1 General Considerations.- 4.2 Fate of Extranuclear Genetic Determinants.- 4.3 Fate of Nuclear Genes in the Process of Somatic Hybridization.- 4.4 Transmission Genetics in Hybridization Systems Utilizing Parental Cell Inactivation.- 4.5 Genetic Diversity of Hybrid Plants Obtained by Protoplast Fusion.- 5 Protoplast Fusion and Hybridization of Distantly Related Plant Species.- 5.1 Introduction.- 5.2 Initial Stages of Culture of Hybrid Cells of Distantly Related Plant Species.- 5.3 Interfamily Cell Hybrids of Higher Plants.- 5.4 Intertribal Hybrids of Higher Plants.- 5.5 Intergeneric Hybrids.- 5.6 Influence of Physical and Chemical Factors on the Fate of Genetic Material in Distantly Related Species Combinations.- 6 Use of Somatic Hybridization.- 6.1 Genetic Analysis Using Parasexual Hybridization.- 6.2 Practical Use of Somatic Cell Hybridization.- 7 Conclusion.- References.

Erscheint lt. Verlag	27.12.2011
Reihe/Serie	Monographs on Theoretical and Applied Genetics
Zusatzinfo	X, 222 p. 53 illus.
Verlagsort	Berlin
Sprache	englisch
Maße	170 x 244 mm
Gewicht	414 g
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Hybride • Pflanzen • Protoplast • Protoplastenfusion
ISBN-10	3-642-82247-9 / 3642822479
ISBN-13	978-3-642-82247-6 / 9783642822476
Zustand	Neuware