Arthropod Cell Cultures and Their Application to the Study of Viruses

1 The Culture of Cells from Insects and Ticks.- I. Cultivation of Dipteran Cells in Vitro.- II. Culture of Cells of the Flesh Fly, Sarcophaga bullata.- III. Establishment and Characterization of Two New Cell Lines (CP-1268 and CP-169) from the Codling Moth, Carpocapsa pomonella (with a Review of Culture of Cells and Tissues from Lepidoptera).- IV. A Cell Line Isolated from Hemocytes of Samia cynthia Pupae.- V. Present Status of Tick Tissue Culture.- 2 Analysis of Cells from Established Insect Cell Lines.- I. Introduction.- II. Morphological and Cultural Characteristics of Culiseta inornata and Aedes vexans Mosquito Cell Lines.- III. Antigenic Relationships of Mosquito Cell Lines as Determined by Immunodiffusion Techniques.- IV. Characterization and Identification of Insect Cell Cultures.- V. Cytogenetic Studies on Cells in Culture from the Class Insecta.- 3 Physiology of Cultivated Arthropod Cells.- I. Introduction.- II. Drosophila Neuron Differentiation in Vitro.- III. Cultivation of Insect Endocrine Glands in Vitro.- IV. Production of Human-Mosquito Somatic Cell Hybrids and their Response to Virus Infection.- V. The Uptake of Free and Serum-Bound Sterols by Insect Cells in Vitro.- VI. Comparative Lipid Analysis of Aedes aegypti and Monkey Kidney Cells (MK-2) Cultivated in Vitro.- VII. Studies Attempting to Elucidate the in Vivo Function(s) of Grace's Antheraea eucalypti Cell Strains.- VIII. The Effects of Insecticides on Insect Cells Grown in Vitro.- 4 Arthropod Tissue Culture in the Study of Arboviruses and Rickettsiae: A Review.- A. Arbovirus Studies.- B. Rickettsial Studies.- C. Concluding Remarks.- 5 Propagation of Arboviruses in Singh's Aedes Cell Lines.- I. Growth of Arboviruses in Aedes albopictus and A. aegypti Cell Lines.- II. Multiplication ofChikungunya and O'nyong-nyong Viruses in Singh's Aedes Cell Lines.- III. Growth Studies of California Encephalitis Virus in Two Aedes Mosquito Cell Line Cultures.- 6 Growth of Arboviruses in Arthropod Cell Cultures: Comparative Studies.- I. Preliminary Observations on Growth of Arboviruses in a Newly Established Line of Mosquito Cell (Culex quinquefasciatusSay).- II. Attempts to Grow Tacaribe and Junin Viruses in Grace's Continuous Line of Moth Cells.- III. A Comparative Study of the Viral Susceptibility of Monolayer and Suspended Mosquito Cell Lines.- 7 Growth of Viruses in Arthropod Cell Cultures: Applications.- I. Attenuation of Semliki Forest (SF) Virus in Continuously Cultured Aedes aegypti Mosquito Cells (Peleg) as a Step in Production of Vaccines.- II. Cultivation of Oncogenic Viruses in Mosquito Cells in Vitro.- III. RNA of Uninfected and Sindbis Virus-Infected Aedes albopictus Cells.- 8 Homoptera Cell Culture and Its Application to the Study of Plant Pathogens.- II. In Vitro Cultivation.- III. Application to the Study of Plant Pathogens.- 9 Lepidoptera Cell Culture and Its Application to the Study of Plant Viruses and Animal Parasites.- I. Cultivation of Gypsy Moth Hemocytes.- II. Growth of a Microsporidian Parasite in Cultured Cells of Tent Caterpillars (Malacosoma).- III. Drosophila Cells in Vitro: Behavior and Utilization for Genetic Purpose.- 10 Drosophila Cell Culture and Its Application for the Study of Genetics and Virology.- I. Drosophila Cells in Vitro: Behavior and Utilization for Genetic Purpose.- II. Established Diploid Cell Lines of Drosophila melanogaster as Potential Material for the Study of Genetics of Somatic Cells.- III. Arbovirus Multiplication in an Established Diploid Cell Line of Drosophila melanogaster.- IV. Sigma VirusMultiplication in Drosophila Cell Lines of Different Genotypes.- V. The SR Condition in Drosophila bifasciata: In Vivo and in Vitro Growth.- 11 New Opportunities in Biological Research Offered by Arthropod Cell Cultures.- I. Some Speculations on the Possible Role of Arthropods in the Evolution of Arboviruses.- II. Future Possibilities of Arthropod Cell Culture in Parasitology and in Developmental Biology.- References.- Author Index.