The Eukaryotic Ribosome

I. Introduction.- References.- A short historical survey.- References.- III. Ribosomes within the cell.- 1. Proportion of ribosomes in tissues.- 2. Free and membrane-bound ribosomes.- 3. Ribosomes in nucleoli.- 4. Structure of polysomes in the cell.- 5. Ribosomal crystals.- 6. References.- IV. Some general properties of ribosomes.- 1. Physical characteristics.- 2. Chemical characteristics.- 3. References.- V. Morphology of ribosomes and polysomes.- 1. Electron microscopy.- 2. Small-angle X-ray scattering.- 3. References.- VI. Chemical components.- 1. Ribosomal proteins.- 2. Phosphorylation of ribosomal proteins.- 3. Immunochemical properties of ribosomal particles and proteins.- 4. Ribosomal ribonucleic acids.- 5. Proteolytic and nucleolytic activities of ribosomal particles.- 6. Other components.- 7. References.- VII. Biosynthesis of ribosomal components and biogenesis of ribosomal particles.- 1. Ribosomal RNA.- 2. Ribosomal proteins.- 3. Formation of preribosomal particles.- 4. References.- VIII. Dissociation-reassociation processes of ribosomal particles.- 1. Dissociation by withdrawal of magnesium.- 2. Other agents for dissociating eukaryotic ribosomes.- 3. Preparation of ribosomal subunits re-associable to active ribosomes.- 4. Reassociation of ribosomal subunits.- 5. Native ribosomal subunits.- 6. References.- IX. Interaction, topography and function of ribosomal components.- 1. Interactions of proteins and RNA.- 2. Topography of proteins and RNA within the ribosome.- 3. Functions of ribosomal proteins.- 4. Functions of ribosomal ribonucleic acids.- 5. References.- X. Interactions of ribosomes with membranes.- 1. Activity of free and membrane-bound polysomes.- 2. Dynamics of ribosomal subunits between free and membrane-bound polysomes.- 3. Chemical properties of free and mem-brane-bound ribosomes.- 4. Components and mechanisms involved in binding of ribosomes to membranes.- 5. Vectorial discharge of polypeptide chains from membrane-bound polysomes.- 6. References.- XI. Function of eukaryotic ribosomes.- 1. Nonribosomal components of the protein synthesizing system.- 2. The process of eukaryotic protein synthesis.- 3. Protein synthesis on membrane-bound ribosomes.- 4. Some regulatory aspects of protein biosynthesis in eukaryotes.- 5. References.- XII. Aphoristic synopsis and outlook.- XIII. Notes added in proof.- XIV. Subject index.- XV. Reference index.