Differential Display Methods and Protocols

Peng Liang, Jonathan Meade, Arthur B. Pardee (Herausgeber)

Buch | Softcover

320 Seiten

2010 | Softcover reprint of hardcover 2nd ed. 2006
Humana Press Inc. (Verlag)
978-1-61737-503-3 (ISBN)

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Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Methodologies.- Global Analysis of Gene Expression by Differential Display.- Automation of Fluorescent Differential Display With Digital Readout.- Ordered Differential Display.- GeneCalling®.- High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR.- Vertical Arrays.- Automated Pattern Ranking in Differential Display Data Analysis.- Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs.- Applications.- Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma.- Identification of Disease Markers by Differential Display.- Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display.- Identification of p53-Regulated Genes by the Method of Differential Display.- Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes.- Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display.- Functional Analysis of Nocturnin.- Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display.- Identification of Target Genes of a Yeast Transcriptional Repressor.- Detection of an mRNA Polymorphism by Differential Display.- Silencing in Yeast.- Identification of mRNA Bound to RNA Binding Proteins by Differential Display.

Erscheint lt. Verlag	9.12.2010
Reihe/Serie	Methods in Molecular Biology ; 317
Zusatzinfo	1 Illustrations, color; 131 Illustrations, black and white; XIV, 320 p. 132 illus., 1 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-503-9 / 1617375039
ISBN-13	978-1-61737-503-3 / 9781617375033
Zustand	Neuware