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Differential Display Methods and Protocols

Peng Liang, Arthur B. Pardee (Herausgeber)

Buch
320 Seiten
1997
Humana Press Inc. (Verlag)
978-0-89603-405-1 (ISBN)
85,59 inkl. MwSt
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This text reveals the current techniques for the identification, isolation, and characterization of differentially expressed genes. It provides a detailed and up-to-date description of general DD methodology, including RAP-PCR, fluorescence detection, and many new modifications.
Drs. Peng Liang and Arthur B. Pardee assemble for the first time a comprehensive review of the state of the art of their powerful new methodology and practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. They also provide numerous examples in which differentially expressed genes were successfully identified in diverse biological systems ranging from plants to songbirds to humans.

Methodologies Differential Display-A General Protocol Peng Liang and Arthur B. Pardee Fingerprinting by Arbitrarily Primed PCR M. McClelland, Rhonda Honeycutt, Francoise Mathieu-Daude, Thomas Vogt, and John Welsh Differential Display Using Random Hexamer-Primed cDNA, Motif Primers, and Agarose Gel Electrophoresis Patrick J. Donohue, Debbie K. W. Hsu, and Jeffrey A. Winkles Fluorescent Differential Display Takashi Ito and Yoshiyuki Sakaki Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization Jackson S. Wan and Mark G. Erlander Direct Sequencing of Differential Display PCR Products Xinkang Wang and Giora Z. Feuerstein A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels Katherine J. Martin, Chi-Pong Kwan, and Ruth Sager Differential Screening of Differential Display cDNA Products by Reverse Northern Hong Zhang, Rong Zhang, and Peng Liang Screening for Positive Clones Generated by Differential Display Regina Vogeli-Lange, Niels Burckert, Thomas Boller, and Andres Wiemken Cloning Family Specific Genes Cloning of the 3' Noncoding Regions From Several Members of Heat Shock Protein Gene Families by Differential Display Chandrashekhar P. Joshi and Henry T. Nguyen RC4D-Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display Gunter Theiben and Achim Fischer Cloning Inducible Genes dentification of Immediate-Early Gene Targets of the Raf-1 Serine/Threonine Protein Kinase Using an Estradiol-Dependent Fusion Protein, DRaf-1:ER Sean A. McCarthy, Natasha Aziz, and Martin McMahon Isolation of Cytokine-Inducible Genes from Hemapoietic Cells by Differential Display Yuan Zhu, Tom Hahn, and Alan D. D'Andrea Hormone-Inducible Genes in Prostate Cells Lidia Averboukh, Peng Liang, Stephen A. Douglas, and Arthur B. Pardee Cloning Differentially Expressed Genes During Normal Development Application of Differential Display in Studying Developmental Processes M. Jorge Guimaraes, Terrill McClanahan, and J. Fernando Bazan Identification of Novel Genes in Adipose Differentiation by Differential Display Erding Hu and Bruce M. Spiegelman Isolation of Song-Regulated Genes in the Brain of Songbirds Claudio V. Mello, Erich D. Jarvis, Natalia Denisenko, and Miriam Rivas Cloning Differentially Expressed Genes in Diseases Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display Carla B. Green and Joseph C. Besharse Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues Mary E. Russell, Anne Raisanen-Sokolowskind, and Ulrike Utans Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model System William D. Pennie, Yi Sun, Hyungtae Kim, and Nancy H. Colburn Identification of Genes Associated With Resistance to Photodynamic Therapy Mediated Oxidative Stress Charles J. Gomer, Marian C. Luna, and Angela Ferrario RAP-PCR Using RNA from Tissue Microdissection Roy A. Jensen, David L. Page, and Jeffrey T. Holt The Application of Differential Display to the Brain: Adaptations for the Study of Heterogeneous Tissue Joseph M. Babity, Richard A. Newton, Mario E. Guido, and Harold A. Robertson Analysis of Gene Expression in Hypothalamus in Obese and Normal Mice Using Differential Display Eleftheria Maratos-Flier, Daqing Qu, and Steen Gammeltoft Index

Erscheint lt. Verlag 7.7.1997
Reihe/Serie Methods in Molecular Biology ; 85
Verlagsort Totowa, NJ
Sprache englisch
Maße 152 x 229 mm
Gewicht 1010 g
Themenwelt Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zoologie
ISBN-10 0-89603-405-4 / 0896034054
ISBN-13 978-0-89603-405-1 / 9780896034051
Zustand Neuware
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