Plant Cell Culture Protocols

An Introduction to Plant Cell Culture.- History of Plant Tissue Culture.- Cell Culture and Plant Regeneration: The Fundamentals.- Pathogen and Biological Contamination Management.- Growth Measurements.- Callus and Suspension Culture Induction, Maintenance, and Characterization.- Measurement of Cell Viability in In Vitro Cultures.- Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification.- Somatic Embryogenesis in Picea Suspension Cultures.- Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes.- Direct Somatic Embryogenesis in Coffea canephora.- PLANT PROPAGATION IN VITRO.- A New Temporary Immersion Bioreactor System for Micropropagation.- Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro.- Use of Statistics in Plant Biotechnology.- An Efficient Method for the Micropropagation of Agave Species.- Micropropagation of Endangered Plant Species.- Clonal Propagation of Softwoods.- Applications for Plant Protoplasts.- Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora.- Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts.- Production of Cybrids in Brassicaceae Species.- Guard Cell Protoplasts.- Production of Interspecific Hybrid Plants in Primula.- Protocols for Genomic Manipulation.- Agrobacterium-Mediated Transformation of Petunia Leaf Discs.- Transformation of Wheat Via Particle Bombardment.- Chloroplast Transformation.- The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers.- Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System.- Accumulation of Metabolites in Plant Cells.- Capsaicin Accumulation in Capsicum spp. Suspension Cultures.- Isolation and Purification of Ribosome-Inactivating Proteins.- Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids.- Methods for Regeneration and Transformation in Eschscholzia californica.