Cytochrome P450 Protocols

Cytochrome P450 Nomenclature, 2004.- Spectral Analyses of Cytochromes P450.- Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli.- Purification of Cytochromes P450.- Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System.- Cytochrome P450 Reconstitution Systems.- Catalytic Assays for Human Cytochrome P450.- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate.- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation.- Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes.- High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6?-Hydroxylation.- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography.- CYP2C19-Mediated (S)-Mephenytoin 4?-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection.- CYP2D6-Dependent Bufuralol 1?-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection.- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation.- Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation.- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection.- An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7?-Hydroxylation.- Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families.- Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity.- ProchiralSulfoxidation as a Probe for Flavin-Containing Monooxygenases.- Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes.- The Human Cytochrome P450 Allele Nomenclature Committee Web Site.- Genotyping for Cytochrome P450 Polymorphisms.- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing.- Isolation of Rat Hepatocytes.- Rat Hepatocyte Cultures.- Rat Hepatocyte Cultures.- Hepatocytes in Suspension.- Isolation of Rat Bone Marrow Stem Cells.- Transfection of Primary Cultures of Rat Hepatocytes.- Human Hepatocyte Culture.- Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization.- Deletion of Genes From the Mouse Genome Using Cre/loxP Technology.- Characterization of Targeted Mouse Embryonic Stem Cell Chromosomes.- Microinjection of Targeted Embryonic Stem Cells and Establishment of Knockout Mouse Lines for Fmo Genes.- Use of Reporter Genes to Measure Xenobiotic-Mediated Activation of CYP Gene Transcription.