Plant Embryo Culture

Covering methodology and protocols, this volume sees expert researchers from the field showing how to culture zygotic embryos for various studies, both theoretical and practical. The book examines a wide range of related topics including cryopreservation.

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A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.