Protocols for Neural Cell Culture

Introduction, Marston Manthorpe. Outgrowth Assays and Cortical Slice Cultures, Valerie Castellani and Jurgen Bolz. Microexplant Cultures of the Cerebellum, Bernard Rogister and Gustave Moonen. Aggregating Neural Cell Cultures, Paul Honegger and Florianne Monnet-Tschudi. Primary Cell Cultures of Peripheral and Central Neurons and Glia, Mary I. Johnson and Richard P. Bunge. Chick Spinal Somatic Motoneurons in Culture, Bernhard H. J. Juurlink. Neural Cells in Microcultures: In Vitro Biological Assays for Neuroactive Agents, Marston Manthorpe. Construction and Use of Compartmented Cultures, Robert B. Campenot. Astrocyte and Oligodendrocyte Cultures, Ruth Cole and Jean de Vellis. Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium, Sergey Fedoroff and Arleen Richardson. Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium, Bernhard H. J. Juurlink, Shawn K. Thorburne, and Richard M. Devon. Culture of Glial Cells from Human Brain Biopsies, Voon Wee Yong and Jack P. Antel. Colony Cultures, Sergey Fedoroff and Arleen Richardson. Herpes Simplex Virus Vectors for Gene Therapy of the Nervous System , Angela P. Dyer and Frank Tufaro. Immunostaining and Identification of Antigens, Colin J. Barnstable. Elimination of Cell Types from Mixed Neural Cell Cultures, Richard M. Devon. Quantification of Cells in Culture, Arleen Richardson and Sergey Fedoroff. Procedures for Subculturing Cells, Arleen Richardson and Sergey Fedoroff. Preparation of Tissue-Cultured Material for Electron Microscopical Observation, Richard M. Devon. Tips in Tissue Culture, Sergey Fedoroff and Arleen Richardson. Use of Dissecting Instruments in Tissue Culture, Arleen Richardson. Abbreviations and Definitions. Index.