Protocols for Neural Cell Culture

In Vitro Assays for Axonal Growth and Targeting Valerie Castellani and Jurgen Bolz Interface Organotypic Hippocampal Slice Cultures Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, and Luc Stoppini Slice Cultures for Study of Microglia Carol A. Colton, Meggan Czapiga, and Toby N. Behar Microexplant Cultures of the Cerebellum Bernard Rogister and Gustave Moonen Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons Robert B. Campenot and Grace Martin Chick Spinal Somatic Motoneurons in Culture Bernhard H. J. Juurlink Primary Cultures of Sympathetic Ganglia Mary I. Johnson Primary Cell Cultures for the Study of Myelination Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures Ruth Cole and Jean de Vellis Culture of Glial Cells from Human Brain Biopsies V. Wee Yong and Jack P. Antel Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium Sergey Fedoroff and Arleen Richardson Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium Bernhard H. J. Juurlink, Shawn K. Thorburne, and Richard M. Devon Olfactory Ensheathing Cell Cultures Ronald Doucette Cultures of Stem Cells of the Central Nervous System Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi Aggregating Neural Cell Cultures Paul Honegger and Florianne Monnet-Tschudi Neural Cell Lines Kaoru Murayama, Natalia N. Singh, Angela Helmrich, and David W. Barnes Virus Vectors for Gene Therapy of the Nervous System Sonia N. Yeung and Frank Tufaro Preparation of Substrata for In Vitro Culture of Neurons Paul C. Letourneau Serum-Fee Media for Neural Cell Cultures: Adult and Embryonic Paul J. Price and Gregory J. Brewer Development of Multiple-Well Plate Biological Assays John W. Harbell Neural Cell Counting: Cell Number Determination in Microplate Cultures Marston Manthorpe Assays for Neuronal Cell Migration Toby N. Behar Colony Culture: Plating Efficiency Assay and Cloning Sergey Fedoroff and Arleen Richardson Hybridoma Cultures for Production of Antibodies: Purification and Specificity of Antibodies J. Denry Sato Elimination of Cell Types from Mixed Neural Cell Cultures Richard M. Devon Quantification of Cells in Culture Arleen Richardson and Sergey Fedoroff Tips for Tissue Culture Arleen Richardson and Sergey Fedoroff