Dengue Virus

Virus Propagation and Plaque Assay for Dengue Virus.- Transmission Electron Microscopy Studies of Dengue Viruses.- Production of Properly Folded NS1 Protein in Bacterial Cells.- Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System.- Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals.- Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly.- Construction of Genomic and Sub-Genomic Dengue Infectious Replicons.- Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis.- Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions.- Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry.- Molecular Diagnosis Of Dengue.- Serological Diagnosis of Dengue.- Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies.- Evaluation of DENV-InducedEndothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus.- In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells.- In Vitro Generation of Human Antibody-Secreting Cells through the Stimulation of PBMCs with Dengue Virus Particles.- Isolation of Microvesicles from Plasma Samples Avoiding Lipoprotein Contamination.- Evaluating Dengue Virus Pathogenesis in Mice and Humans by Histological and Immunohistochemistry Approaches.- Humanized Mice for the Study of Dengue Disease Pathogenesis: Biological Assays.