Dengue Virus
Springer-Verlag New York Inc.
978-1-0716-1878-3 (ISBN)
Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.
Virus Propagation and Plaque Assay for Dengue Virus.- Transmission Electron Microscopy Studies of Dengue Viruses.- Production of Properly Folded NS1 Protein in Bacterial Cells.- Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System.- Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals.- Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly.- Construction of Genomic and Sub-Genomic Dengue Infectious Replicons.- Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis.- Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions.- Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry.- Molecular Diagnosis Of Dengue.- Serological Diagnosis of Dengue.- Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies.- Evaluation of DENV-InducedEndothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus.- In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells.- In Vitro Generation of Human Antibody-Secreting Cells through the Stimulation of PBMCs with Dengue Virus Particles.- Isolation of Microvesicles from Plasma Samples Avoiding Lipoprotein Contamination.- Evaluating Dengue Virus Pathogenesis in Mice and Humans by Histological and Immunohistochemistry Approaches.- Humanized Mice for the Study of Dengue Disease Pathogenesis: Biological Assays.
Erscheinungsdatum | 05.11.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2409 |
Zusatzinfo | 56 Illustrations, color; 9 Illustrations, black and white; XI, 295 p. 65 illus., 56 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Cellular proteins • DENV • Immunopathogenesis • serotypes • Vaccine development • Viral Replication • Virus protein production |
ISBN-10 | 1-0716-1878-4 / 1071618784 |
ISBN-13 | 978-1-0716-1878-3 / 9781071618783 |
Zustand | Neuware |
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