Molecular Diagnosis of Cancer

Part I: Hematological malignancies. PCR of Gene Rearrangements for the Detection of Minimal Residual Disease in Childhood ALL. Detection of BCR-ABL in Hematological Malignancies by RT-PCR. Molecular Diagnosis of Acute Myeloid Leukemia with Maturation FAB- Type M2. Analysis of the PML/RAR-a Fusion Gene in Acute Promyelocytic Leukemia by Reverse- Transcription Polymerase Chain Reaction. RT-PCR Analysis of Breakpoints Involving the MLL Gene Located at 11q23 in Acute Leukemia. Polymerase Chain Reaction for Detection of the t(14;18) Translocation in Lymphomas. NPM-ALK Reverse Transcriptase-Polymerase Chain Reaction Analysis for Detection of the t(2;5) Translocation of Non-Hodgkin's Lymphoma. Molecular Diagnosis of the 5q Deletion in Malignant Myeloid Disorders. Polymerase Chain Reaction Based Methods for Assessing Chimerism Following Allogeneic Bone Marrow Transplantation. Part II: Solid Tumors. Identification of Mutations in the Retinoblastoma Gene. Mutational Analysis of the Wilms Tumor (WT1) Gene. Part III: General Techniques for Cancer Analysis. Single-Strand Conformation Polymorphism Mutation Analysis of the p53 Gene. The Characterization of Chromosomal Abnormalities Using Fluorescence In Situ Hybridization Procedures. Comparative Genomic Hybridization. In Situ Hybridization of Cells and Tissue Sections. Apoptosis Detection by DNA Analysis. Index.