Practical Methods in Cardiovascular Research
Springer Berlin (Verlag)
978-3-540-40763-8 (ISBN)
In-Vivo Techniques.- Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animals.- Extracorporeal Circulation.- Open-chest Models of Acute Myocardial Ischemia and Reperfusion.- Models of Chronic Ischemia and Infarction.- Models on Left Ventricular Hypertrophy (LVH).- Experimental Models of Heart Failure.- In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Death.- In-Vivo Models of Atrial Fibrillation.- In-Vitro Techniques.- The Langendorff Heart.- The Working Heart.- Isolated Papillary Muscles.- Isolated Vessels.- Optical Techniques for the Recording of Action Potentials.- Recording Action Potentials Using Voltage-Sensitive Dyes.- Epicardial Mapping in Isolated Hearts - Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmia.- Voltage-Clamp and Patch-Clamp Techniques.- L-Type Calcium Channel Recording.- Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Technique.- Recording the Pacemaker Current If.- Recording Gap Junction Currents.- Recording Monophasic Action Potentials.- Heart on a Chip - Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitro.- Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research.- Classical Histological Staining Procedures in Cardiovascular Research.- Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Research.- Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research.- DAF Technique for Real-Time NO Imaging in the Human Myocardium.- Isolation and Culture of Adult Ventricular Cardiomyocytes.- Culture of Neonatal Cardiomyocytes.- Culture of Embryoid Bodies.- Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapy.- Preparation of Endothelial Cells from Microand Macrovascular Origin.- In Vitro Cultivation of Vascular Smooth Muscle Cells.- Engineering Heart Tissue for In Vitro and In Vivo Studies.- High-Performance Liquid Chromatography (HPLC).- Protein Biochemistry.- Receptor and Binding Studies.- Recording and Analysing Concentration-Response Curves.- Measurement of NO and Endothelial Function in Cardiovascular Research.- Biomechanical Stimulation of Vascular Cells In Vitro.- Measurement of Function and Regulation of Adrenergic Receptors.- Measurement of Function and Regulation of Muscarinic Acetylcholine Receptors.- Cytometry in Cardiovascular Research.- Molecular Biology in Cardiovascular Research.- Real-Time PCR Analysis of Cardiac Samples.- Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Lines.- Using Antibody Arrays to Detect Protein-Protein Interactions.- Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein- Protein Binding.- How to Solve a Protein Structure by Nuclear Magnetic Resonance - The Connexin43 Carboxyl Terminal Domain.- Managing a Transgenic Mouse Colony: A Guide for the Novice.
Erscheint lt. Verlag | 14.12.2004 |
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Zusatzinfo | XX, 1010 p. 231 illus., 31 illus. in color. |
Verlagsort | Berlin |
Sprache | englisch |
Maße | 155 x 235 mm |
Gewicht | 1555 g |
Themenwelt | Medizinische Fachgebiete ► Innere Medizin ► Kardiologie / Angiologie |
Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin | |
Schlagworte | anesthesia • Atrial Fibrillation • Cardiovascular • Circulation • heart • Kardiologie • Laboratory • sudden cardiac death • Transplantation |
ISBN-10 | 3-540-40763-4 / 3540407634 |
ISBN-13 | 978-3-540-40763-8 / 9783540407638 |
Zustand | Neuware |
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