Practical Methods in Cardiovascular Research (eBook)

In-Vivo Techniques.- Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animals.- Extracorporeal Circulation.- Open-chest Models of Acute Myocardial Ischemia and Reperfusion.- Models of Chronic Ischemia and Infarction.- Models on Left Ventricular Hypertrophy (LVH).- Experimental Models of Heart Failure.- In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Death.- In-Vivo Models of Atrial Fibrillation.- In-Vitro Techniques.- The Langendorff Heart.- The Working Heart.- Isolated Papillary Muscles.- Isolated Vessels.- Optical Techniques for the Recording of Action Potentials.- Recording Action Potentials Using Voltage-Sensitive Dyes.- Epicardial Mapping in Isolated Hearts — Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmia.- Voltage-Clamp and Patch-Clamp Techniques.- L-Type Calcium Channel Recording.- Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Technique.- Recording the Pacemaker Current If.- Recording Gap Junction Currents.- Recording Monophasic Action Potentials.- Heart on a Chip — Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitro.- Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research.- Classical Histological Staining Procedures in Cardiovascular Research.- Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Research.- Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research.- DAF Technique for Real-Time NO Imaging in the Human Myocardium.- Isolation and Culture of Adult Ventricular Cardiomyocytes.- Culture of Neonatal Cardiomyocytes.- Culture of Embryoid Bodies.- Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapy.- Preparation of Endothelial Cells from Microand Macrovascular Origin.- In Vitro Cultivation of Vascular Smooth Muscle Cells.- Engineering Heart Tissue for In Vitro and In Vivo Studies.- High-Performance Liquid Chromatography (HPLC).- Protein Biochemistry.- Receptor and Binding Studies.- Recording and Analysing Concentration-Response Curves.- Measurement of NO and Endothelial Function in Cardiovascular Research.- Biomechanical Stimulation of Vascular Cells In Vitro.- Measurement of Function and Regulation of Adrenergic Receptors.- Measurement of Function and Regulation of Muscarinic Acetylcholine Receptors.- Cytometry in Cardiovascular Research.- Molecular Biology in Cardiovascular Research.- Real-Time PCR Analysis of Cardiac Samples.- Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Lines.- Using Antibody Arrays to Detect Protein-Protein Interactions.- Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein- Protein Binding.- How to Solve a Protein Structure by Nuclear Magnetic Resonance — The Connexin43 Carboxyl Terminal Domain.- Managing a Transgenic Mouse Colony: A Guide for the Novice.