In Vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials

Toru Takebayashi Keio University Graduate School of Health Management Fujisawa, Kanagawa, Japan Robert Landsiedel BASF SE Experimental Toxicology and Ecology Ludwigshafen am Rhein, Germany Masashi Gamo National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba, Ibaraki, Japan

1 The role of in vivo screening studies in assessing manufactured nanomaterials.- Part 1 Short Term Inhalation Study.- 2. The short-term inhalation study (STIS) as a range finder and screening tool in a tiered grouping strategy.- 3. Use of short-term inhalation study to obtain initial hazard data and prepare for subacute and subchronic inhalation studies, and toxicokinetic studies.- 4. Subchronic inhalation Toxicity Study with a Vapor Grown Carbon Nanofiber in Male and Female Rats (OECD 413) – Does nanofiber exposure have adverse impacts on the cardiovascular system?.- Part 2 Intratracheal Administration Study.- 5. Comparison of responses in rat lung following inhalation and intratracheal administration of nanoparticles.- 6. Standardization of intratracheal instillation study of manufactured nanomaterials.- 7. Sample preparation and the chracterization for Intratracheal Administration.- 8. Development of intra-tracheal intra-pulmonary spraying (TIPS) as an alternative assay method for testing chronic toxicity and carcinogenic potential of multiwall carbon nanotubes.- 9. Equivalence Criteria for Nanomaterials Developed from Results of a Comparative Study using Intratracheal Administration.- 10. Toxicokinetics of nano materials after the intratracheal administration.- 11. In Vitro Alveolar Epithelial Models toward the Prediction of Cytotoxicity Tests and Translocation Studies of Nanoparticles.