Basic Methods In Molecular Biology

General methods; bacterial strains and cloning vectors; enzymes that modify DNA and RNA; in vitro amplification of DNA using - the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction; DNA restriction fragment analysis and preparation, introduction; in vitro labeling of probes and filter hybridization; plasmid DNA preparation for E. Coli hosts; preparation of DNA from Lambda bacteriophage clones; subcloning fragments into plasmid vectors and plasmid construction; preparation of genomic DNA; preparation and analysis of RNA from eukaryotic cells - overview; geonomic cloning; generatino of cDNA libaries; preparation of subtractive cDNA, introduction; chain termination sequencing; transfection of mammalian cells, introduction; basic methods of protein analysis; in situ hybridization; transgenic mouse preparation; detection and in vitro generation of specific mutations in genes and cDNAs, introduction; appendices.