Veterinary Clinical Parasitology (eBook)

Anne M. Zajac, DVM, PhD, is Associate Professor in theDepartment of Biomedical Sciences and Pathobiology atVirginia-Maryland Regional College of Veterinary Medicine, VirginiaTech, Blacksburg, Virginia. Gary A. Conboy, DVM, PhD, is Associate Professor in theDepartment of Pathobiology and Microbiology at Atlantic VeterinaryCollege, University of Prince Edward Island, Charlottetown, PrinceEdward Island, Canada.

Preface vii

Acknowledgments ix

Authors xi

Chapter 1 Fecal Examination for the Diagnosis of Parasitism 3

Collection of Fecal Samples 3

Storage and Shipment of Fecal Samples 4

Fecal Exam Procedures 4

Fecal Flotation 4

Additional Procedures for Fecal Examination 12

Quality Control for Fecal Exam Procedures 15

Use of the Microscope 16

Microscope Calibration 16

Pseudoparasites and Spurious Parasites 19

Identification of Nematode Larvae Recovered with Fecal Flotation or Baermann Procedures 23

Techniques for Evaluation of Strongylid Nematodes in Grazing Animals 28

Fecal Culture 28

Identification of Ruminant and Camelid Third-Stage Larvae 29

Identification of Horse Third-Stage Larvae 30

Fecal Egg Count Reduction Test 30

Identification of Adult Worms 38

Hoyer's Solution 39

Lactophenol 39

Parasites of Domestic Animals 39

Dogs and Cats 40

Ruminants and Camelids 88

Horses 114

Swine 128

Birds 140

Rodents and Rabbits 156

Reptiles 164

Chapter 2 Detection of Protozoan and Helminth Parasites in the Urinary, Reproductive, and Integumentary Systems and in the Eye 171

Techniques for Parasite Recovery 171

Parasites of the Urinary System 171

Parasites of the Reproductive Tract 171

Helminth Parasites of the Integumentary System 172

Urinary System Parasites 174

Reproductive System Parasites 178

Parasites of Other Systems (Excluding Arthropods) 178

Chapter 3 Detection of Parasites in the Blood 185

Immunologic Detection of Blood Parasites 185

Microscopic Examination of Blood for Protozoan Parasites 185 Giemsa Stain 186

Microscopic Examination of Blood for Nematode Parasites 187

Tests for Canine Heartworm Microfilariae in Blood Samples 188

Blood Parasites of Dogs and Cats 191

Blood Parasites of Livestock and Horses 202

Blood Parasites of Birds 208

Chapter 4 Immunodiagnostic and Molecular Diagnostic Tests in Veterinary Parasitology 213

Immunodiagnostic Methods 213

Molecular Diagnostic Methods 216

Chapter 5 Diagnosis of Arthropod Parasites 217

Subclass Acari (Mites and Ticks) 217

Mite Identification 217

Tick Identification 246

Class Insecta 264

Lice (Orders Anoplura and Mallophaga) 266

Fleas (Order Siphonaptera) 280

Flies (Order Diptera) 288

Other Insects 302

Chapter 6 Parasites of Fish 305

Techniques for Recovery of Ectoparasites 305

Skin Biopsy (Mucus Smear) 305

Fin Biopsy (Fin Snip) 306

Gill Biopsy (Gill Snip) 307

Recovery of Endoparasites 307

Parasites of Fish 308

Bibliography 325

Index 327

"This book is one which the reviewer can see as being veryuseful both in the veterinary technology classroom as well as onthe bench of a working laboratory." (VeterinaryInformation Network, 9 January 2013)

"Overall, an excellent reference book with outstandingimages and aids to parasite identification. I have utterly enjoyedthe opportunity to review this book and will value its addition toour lab bookshelf ." (Veterinary Record,15 June 2013)

"This update would be an excellent resource for anyone involvedin veterinary parasitology, practitioners as well as students. Itprovides practical, useful, and up-to-dateinformation." (Doody's, 17 August2012)