Veterinary Hematology and Clinical Chemistry (eBook)

THE EDITORS Mary Anna Thrall, DVM, MS, DACVP, is a Professor and Section Chief, Department of Pathobiology at Ross University School of Veterinary Medicine in Basseterre, St. Kitts, West Indies. Glade Weiser, DVM, DACVP, is a Professor, Special Appointment, and Clinical Pathologist in the Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences at Colorado State University in Fort Collins, Colorado. Robin W. Allison, DVM, PhD, DACVP, is an Associate Professor in the Department of Veterinary Pathobiology, Center for Veterinary Health Sciences at Oklahoma State University in Stillwater, Oklahoma. Terry W. Campbell, DVM, PhD, is a Professor in the Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, at Colorado State University in Fort Collins, Colorado.

Contributors ix

Preface x

Preface to the First Edition xii

Section I General Principles of Laboratory Testing and Diagnosis 1

1 Laboratory Technology for Veterinary Medicine 3
Glade Weiser

2 Sample Collection Processing and Analysis of Laboratory Service Options 34
Glade Weiser

3 Perspectives in Laboratory Data Interpretation and Disease Diagnosis 40
Glade Weiser and Robin W. Allison

4 Immunodiagnostics: Current Use and Future Trends in Veterinary Medicine 51
Wayne A. Jensen

Section II Hematology of Common Domestic Species 59

5 Erythrocyte Morphology 61
Mary Anna Thrall

6 Classification of and Diagnostic Approach to Anemia 75
Mary Anna Thrall

7 Nonregenerative Anemia 81
Mary Anna Thrall

8 Regenerative Anemia 87
Mary Anna Thrall

9 Classification of and Diagnostic Approach to Polycythemia 114
Mary Anna Thrall

10 Introduction to Leukocytes and the Leukogram 118
Glade Weiser

11 Neutrophil Production Trafficking and Kinetics 123
Glade Weiser

12 Interpretation of Leukocyte Responses in Disease 127
Glade Weiser

13 Molecular Diagnostics of Hematologic Malignancies 140
Anne Avery

14 Laboratory Evaluation of Bone Marrow 150
Mary Anna Thrall and Glade Weiser

15 Lymphoproliferative Disorders and Myeloid Neoplasms 166
Mary Anna Thrall

16 Diagnosis of Disorders of Hemostasis 185
Dale C. Baker

17 Principles of Blood Transfusion and Crossmatching 205
Diane Brown and Linda M. Vap

Section III Hematology of Common Nondomestic Mammals Birds Reptiles Fish and Amphibians 223

18 Mammalian Hematology: Laboratory Animals and Miscellaneous Species 225
Terry W. Campbell

19 Hematology of Birds 238
Terry W. Campbell

20 Hematology of Reptiles 277
Terry W. Campbell

21 Hematology of Fish 298
Terry W. Campbell

22 Hematology of Amphibians 313
Terry W. Campbell

Section IV Clinical Chemistry of Common Domestic Species 321

23 Laboratory Evaluation and Interpretation of the Urinary System 323
Donald Meuten

24 Laboratory Evaluation of Electrolytes 378
Andrea A. Bohn

25 Laboratory Evaluation of Acid-Base Disorders 393
Glade Weiser

26 Laboratory Evaluation of the Liver 401
Robin W. Allison

27 Laboratory Evaluation of the Pancreas and Glucose Metabolism 425
Robin W. Allison

28 Laboratory Evaluation of Digestion and Intestinal Absorption 441
Dawn Seddon

29 Laboratory Evaluation of Plasma and Serum Proteins 460
Robin W. Allison

30 Laboratory Detection of Muscle Injury 476
Robin W. Allison

31 Laboratory Evaluation of Lipids 480
M. Judith Radin

32 Laboratory Evaluation of the Thyroid Adrenal and Pituitary Glands 497
Donald Meuten

33 Parathyroid Glands and Calcium and Phosphorus Metabolic Pathology 545
Donald Meuten

Section V Clinical Chemistry of Common Nondomestic Mammals Birds Reptiles Fish and Amphibians 569

34 Chemical Chemistry of Mammals: Laboratory Animals and Miscellaneous Species 571
Terry W. Campbell

35 Clinical Chemistry of Birds 582
Terry W. Campbell

36 Clinical Chemistry of Reptiles 599
Terry W. Campbell

37 Clinical Chemistry of Fish and Amphibians 607
Terry W. Campbell

Section VI Clinical Case Presentations 615

This section includes 74 cases with clinicopathologic data accompanied by an interpretive discussion and diagnostic summary.

Index 750

Extra PowerPoint presentations available online at www.wiley.com/go/thrall.

"It will also prove to be of benefit for clinical
pathology residents and specialists who deal with nondomestic and
exotic species." (Journal of The American Veterinary
Medical Association, 15 March 2013)

"I found the self-study part really useful . . . A great
investment to help you decipher the mysteries of the lab
reports." (Tomorrow's vets, 1 September
2013)

"This book cannot be highly recommended enough to the vet
student, and I believe it is just as indispensable for the
veterinary surgeon in practice - no mean feat for a
textbook." (Veterinary Practice News, 1
September 2013)

"In summary, this is a comprehensive text with a strong
practical and clinical focus and. It's clearly written and
supplemented by generous numbers of quality images . . . This book
has a small animal bias and as such will be of greatest interest to
small animal clinicians, but will also be valuable to clinicians
seeing mixed practice, or delving into providing small mammal or
exotic pet clinical pathology." (Australian
Veterinary Journal, 9 September 2013)

"The second edition certainly fulfils its stated objective
and is a valuable resource for its targeted audience. It will
also prove be benefit for clinical pathology residents and
specialists who deal with nondomestic and exotic
species." (Journal of the American Veterinary
Medical Association, 15 March 2013)
"Overall, I think this book is well written and beautifully
presented. It appears to bridge the gap between undergraduate
and postgraduate readers and covers all the species likely to be
encountered in mixed veterinary practice."
(Veterinary Record, 16 March 2013)

"Mary Anna Thrall, et.al.'s VETERINARY HEMATOLOGY AND CLINICAL
CHEMISTRY, 2ND EDITION (9780813810270, $99.99) offers a key to
understanding veterinary laboratory diagnostic techniques and
interpretation and covers hematology and chemistry for a range of
species to include fish, amphibians and birds. This second edition
includes many revisions and additions, from new chapters on
molecular diagnostics of hematologic malignancies to updates in
advancements on diagnostic technology and revisions to data
interpretation. Both elementary basics and advanced laboratory
techniques are covered in a guide that offers over seventy case
studies to help readers understanding the entire laboratory
process, making for a top recommendation for veterinary reference
collections." (Midwest Book Reviews, 1 December
2012)
"It's a powerful reference packed with practical applications and
should be considered a 'must' for any veterinary reference
library." (Midwest Book Reviews, 1 November 2012)