Practical in Situ Hybridization

In situ hybridization is a powerful tool used in cell and molecular biology and can be used to localize and identify nucleic acid sequences (DNA and RNA) within the compartments of the cell. "In Situ Hybridization" is for those who wish to learn and use efficient and reliable protocols in their work and at researchers who wish to check the validity and interpretation of published data. It is a user- friendly, detailed guide for laboratory use. It offers comprehensive coverage of many in situ techniques and has full color illustrations which show results clearly. TOC: The in situ hybridization experiment ** the in situ hybridization laboratory ** the probe ** Labelling the probe ** Chromosome slide preparation ** DNA fibre spreading ** Hybridization ** Oligomer probe hybridization ** Primer extension ** Detection and imaging ** Electron microscopic in situ hybridization ** in solution hybridization ** RNA hybridization ** troubleshooting ** the future ** references ** standard buffers and solutions ** suppliers