DNA Manipulation and Analysis
Springer-Verlag New York Inc.
978-1-0716-3006-8 (ISBN)
Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.
Classical Recombinant DNA Cloning.- A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector.- Molecular cloning using in vivo DNA assembly.- Assembling multiple fragments: The Gibson Assembly.- TA cloning approaches to cloning DNA with damaged ends DNA.- PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis.- Random Mutagenesis by PCR.- In vitro Site Directed Mutagenesis.- Xenopus transgenesis using the pGateway system.- CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog.- A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation.- A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets. -Generation of functional-RNA arrays by in vitrotranscription and in situ RNA capture for the detection of RNA-RNA interactions.- Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry.- Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products.- Next generation Sequencing for mixed samples.- Ethics, Legality and Safety for Geneticists.
Erscheinungsdatum | 06.03.2024 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 44 Illustrations, color; 5 Illustrations, black and white; X, 249 p. 49 illus., 44 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Informatik ► Weitere Themen ► Bioinformatik |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | Capillary electrophoresis • cDNA libraries • CRISPR-Cas9 • DNA Aptamers • plasmid cloning • Site specific Recombinases • Xenopus |
ISBN-10 | 1-0716-3006-7 / 1071630067 |
ISBN-13 | 978-1-0716-3006-8 / 9781071630068 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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