DNA Manipulation and Analysis

Classical Recombinant DNA Cloning.- A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector.- Molecular cloning using in vivo DNA assembly.- Assembling multiple fragments: The Gibson Assembly.- TA cloning approaches to cloning DNA with damaged ends DNA.- PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis.- Random Mutagenesis by PCR.- In vitro Site Directed Mutagenesis.- Xenopus transgenesis using the pGateway system.- CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog.- A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation.- A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets. -Generation of functional-RNA arrays by in vitrotranscription and in situ RNA capture for the detection of RNA-RNA interactions.- Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry.- Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products.- Next generation Sequencing for mixed samples.- Ethics, Legality and Safety for Geneticists.