Methods in Cell Wall Cytochemistry

Krishnamurthy, K V

Preface
Cell Wall-Morphology & Chemical Composition
Carbohydrates
Cellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated Polysaccharides
Glycoproteins
Structural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like Proteins
Enzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and Ribonucleuses
Lipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and Tryphine
Lignin and Other Phenolic Substances-Lignins, Phenolic Acids and Amides
Mineral Substances-Silica, Calcium, and Boron
Light Microscopic Cytochemistry
Introduction
Tissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of Materials
Stains and Staining-Introduction, Control Procedures, Incubation of Materials
Mounting of Stained Materials
Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing Procedures
Procedures for the Cytochemical Localizations of Different Cell Wall Substances
Carbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharides
Proteins-Non-Specific Cell Wall Proteins, Enzymic Proteins
Lignins
Sporopollenins
Cutins & Suberin
Minerals
Fluorescent Microscopic Cytochemistry
Introduction
Fluorescent Cytochemical Methods
Cell Wall Carbohydrates
Cell Wall Proteins
Lignin
Ferulic Acid
Sporopollenin
Cutin and Suberin
Transmission Electron Microscopic Cytochemistry
Introducti