Zebrafish: Cellular and Developmental Biology, Part B (eBook)

Cover 1
Methods in Cell Biology 4
Copyright 5
Dedication 6
Contents 8
Contributors 12
Preface 14
Chapter 1 - Live Imaging of the Cytoskeleton in Early Cleavage-Stage Zebrafish Embryos 16
Abstract 16
Introduction 17
Maintaining the Breeding Competence of Zebrafish throughout the Day 19
Mounting Zebrafish Embryos for Live Imaging 20
Live Imaging of Microtubules in Cleaving Zebrafish’Embryos 21
Live Imaging of Microfilaments in Cleaving Zebrafish’Embryos 25
Comparison of Microscopic Techniques for Imaging the Cytoskeleton of Cleaving Zebrafish Embryos 27
Discussion and Future Directions 29
Acknowledgments 30
Appendix A 30
Supplementary Movies 30
References 30
Chapter 2 - Analysis of Cell Proliferation, Senescence, and Cell Death in Zebrafish Embryos 34
Abstract 34
Introduction: The Cell Cycle in Zebrafish 35
Zebrafish Embryo Cell-Cycle Protocols11Items in boldface indicate reagents and supplies listed in Section V. 37
Screening for Chemical Suppressors of Zebrafish Cell-Cycle Mutants 46
Conclusions 49
Reagents and Supplies 49
Acknowledgments 50
References 50
Chapter 3 - Analysis of Cilia Structure and FunctioninZebrafish 54
Abstract 55
Introduction 55
Cilia in Zebrafish Embryos and Larvae 56
Analytical Tools for Cilia Morphology and Motility 62
Analysis of Cilia-related Mutant Phenotypes in Zebrafish 69
Future Directions 83
Acknowledgments 84
References 84
Chapter 4 - Cellular Dissection of Zebrafish Hematopoiesis 90
Abstract 90
Introduction 91
Zebrafish Hematopoiesis 91
Hematopoietic Cell Transplantation 101
Enrichment of Hematopoietic Stem Cells 109
In vitro Culture and Differentiation of Hematopoietic Progenitors 112
Conclusions 120
References 120
Chapter 5 - Zebrafish Lipid Metabolism: From Mediating Early Patterning to the Metabolism of Dietary Fat and Cholesterol 126
Abstract 126
Abbreviations 127
Introduction 127
Lipid Metabolism in Developing Zebrafish 129
Yolk Metabolism During Early Vertebrate Development 130
Lipid Signaling During Early Zebrafish Development 133
Visualizing Lipid Metabolism in Larval and Adult Zebrafish 137
Triple Screen: Phospholipase, Protease and Swallowing Function Assays 146
Zebrafish Models of Human Dyslipidemias 149
Summary 150
Acknowledgments 150
References 151
Chapter 6 - Development of the Zebrafish Enteric Nervous System 158
Abstract 158
Introduction 159
Organization of the Zebrafish Intestinal Tract 159
Early Development of the ENS 161
Genetic Approaches to Studying ENS Development 163
Molecular Mechanisms of ENS Development 164
ENS Differentiation 165
Regulation of Gut Motility 166
Zebrafish ENS as a Model for Understanding Human Diseases 168
Future Prospects 170
Acknowledgments 171
References 171
Chapter 7 - A Guide to Analysis of Cardiac Phenotypes in the Zebrafish Embryo 176
Abstract 176
Introduction 176
Defects in Heart Size 178
Defects in Heart Shape 183
Defects in Cardiac Function 189
Summary 191
References 191
Chapter 8 - Chemical Approaches to Angiogenesis in•Development and Regeneration 196
Abstract 196
Introduction 197
Chemical Approaches and Zebrafish Vascular Development 198
Novel Chemical Strategies to Investigate Mechanisms of Angiogenesis 204
Summary 207
Acknowledgments 207
References 207
Chapter 9 - Laser-Induced Thrombosis in Zebrafish 212
Abstract 212
Introduction 212
Vascular Occlusion 214
Methods 214
Future Perspectives 217
References 218
Chapter 10 - Endoderm Specification, Liver Development, and Regeneration 220
Abstract 220
Review of the Literature 221
Embryonic and Larval Protocols to Analyze Liver Formation 225
Liver Injury and Regeneration Protocols 228
Assessment of Liver Function 232
Summary 234
References 234
Chapter 11 - Morphogenesis of the Zebrafish Jaw: Development Beyond the Embryo 240
Abstract 240
Postembryonic Development & ndash
Obtaining Phenotypes 243
Quantitative Methods for Studying Adult Phenotypes 246
A Complementary Approach: The Use of Natural Variation to Complement that Generated in the Lab for Understanding Jaw Morphogenesis 257
Implications and Conclusions 259
References 260
Chapter 12 - Associative Learning in Zebrafish (Danio rerio) 264
Abstract 264
Introduction 265
Rationale 266
Methods and Discussion 268
Summary 283
Acknowledgments 283
References 283
Index 286
Volumes in Series 298
Color Plates 308