Bacteria from Fish and Other Aquatic Animals

Dr Nicky Buller has been the laboratory supervisor of the bacteriology laboratory at the Department of Primary Industries and Regional Development in Perth, Western Australia for over 30 years. Bacteria from terrestrial and aquatic animals are identified using phenotypic identification (conventional biochemical methods and MALDI-TOF), and molecular methods (PCR and sequencing). Dr Buller has trained scientists in aquatic bacteriology methods from Australia, New Zealand, Hong Kong, Vietnam and French Polynesia and has given workshops on aquatic bacteria identification methods to students, including overseas students, at Curtin University as part of a Post Graduate degree in aquaculture management. Dr Buller is also author or co-author of 43 journal articles, 7 Australian and New Zealand Standard Diagnostic Procedures and many papers at conferences. She has been Principal or Co-investigator on 11 research projects investigating terrestrial and aquatic animal diseases, including supervision of PhD, Masters or Honours students. She is a reviewer for 16 journals and holds an adjunct position with Murdoch University.

1: Foreword, J A Plumb 2: Aquatic Animal Species and Organism Relationship 3: Host species: Bacteria and Disease 4: Bacterial Diseases 5: Bacteria and Relationship to Host 6: Taxonomy and Disease Status of Bacteria 7: Bacteriological Culture Techniques 8: Specimen Collection and Submission 9: Culture and Incubation 10: Examination of Culture Plates 11: Biochemical Identification Tests 12: Inoculation of Biochemical Identification Sets 13: API Identification Systems 14: Interpretation of Biochemical Identification Tests and Sets 15: Conventional Media - "Biochem Set" 16: Identification Tests and their Interpretation 17: Using the Biochemical Identification Tables 18: Interpretation and Identification of Genera and Species 19: Antisera available 20: Biochemical Identification Tables 21: Results for Conventional Biochemical Tests - "Biochem Set" 22: Results for API Kits 23: Technical Methods 24: Total Bacterial Count 25: Microscopy 26: Storage of Isolates 27: Techniques for the Molecular Identification of Bacteria 28: Molecular Identification by PCR using Specific Primers 29: PCR Protocols 30: Molecular Identification by 16S rDNA Sequencing. 31: Fluorescent In Situ Hybridization (FISH) 32: Preparation of Media for Culture and Identification 33: General Isolation and Selective Media 34: Biochemical Test Media 35: Further Reading and other Information Sources 36: Appendix: Common Name and Scientific Name of Aquatic Animals 37: Glossary of terms 38: References 39: Index