Protocols in Semen Biology (Comparing Assays)

Dr. N Srivastava is a Senior Scientist (Agricultural Research Services) in the ICAR- Central Institute for Research on Cattle, in Meerut, India. Previously, he was working with the ICAR- Indian Veterinary Research Institute, in Izatnagar, India. His specialization is in Animal Reproduction and Gynaecology. He has over 52 research papers in high rated journals and 9 books, besides several popular articles to his credit. He is a part of a number of scientific societies, namely, Indian Science Congress, Indian Society for Study of Animal Reproduction, Indian Society for Study of Reproduction and Fertility, Research Board of Advisors of American Biographical Institute, Indian Veterinary Association, A & N Chapter, Port Blair, Indian Academy of Veterinary Nutrition and Animal Welfare etc. He has also received a number of awards including Merit Scholarships, National Eligibility Test and three Gold Medals in the field of Animal Reproduction. Dr. Megha Pande, is scientist (Agricultural Research Services) of repute working in the Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, in Meerut, India. Her specialization is in Animal Reproduction and Gynaecology. She has published over 23 research papers in high rated journals and 2 training manuals, besides several popular articles to her credit. She is an active member of a number of scientific societies in her field, namely, Indian Society for Study of Reproduction and Fertility, Indian Science Congress, Indian Society for Study of Animal Reproduction etc. She has also received a number of awards in her academic field including Gold (Graduation) and Silver (Post-Graduation) medal, besides qualifying National Eligibility Test and Senior Research Fellow.

Chapter 1. Semen Analysis – An Overview.- Chapter 2. Selection of Spermatozoa.- Chapter 3. Handling and Applications of Optical Devices.- Chapter 4. Basic Semen Assays.- Chapter 5. Estimates of Sperm Motility.- Chapter 6. Evaluating Sperm Cell Viability & Membrane Integrity.- Chapter 7. Counting Sperm Numbers.- Chapter 8. Evaluating Sperm Cell Morphology.- Chapter 9. Biochemical Assays in Spermatology.- Chapter 10. Estimating Metabolic Activity of Spermatozoa.- Chapter 11. Evaluating Resistance of Spermatozoa to Adverse Conditions.- Chapter 12. Determining Oxidative Stress of Spermatozoa.- Chapter 13. Determination of Mitochondrial Function in Sperm Cells.- Chapter 14. Determination of Capacitation Related Changes Using Fluorescent Stains.- Chapter 15. Evaluating Damages to Sperm DNA.- Chapter 16. Estimating Proteins of Semen.- Chapter 17. Measuring Lipids of Spermatozoa and Seminal Plasma.- Chapter 18. Gel Electrophoresis of Proteins and Nucleic Acids.- Chapter 19. Immuno-Reproductive Assays in Semen Biology.- Chapter 20. Sperm Function Assays.