Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea

Enteric Viruses.- Papers presented.- Viral agents associated with neonatal diarrhoea and their detection by electron microscopy..- Cell culture techniques for the identification of enteric viruses — present possibilities..- Diagnosis by enzyme-linked immunosorbent assay..- Application of purified 5-aminosalicylic acid as substrate in enzyme-linked immunosorbent assay (ELISA)..- ELISA for the detection of rotavirus and rotavirus/antibody complexes in faeces..- RNA gel electrophoresis: a technique to monitor laboratory cross-contaminations and genetic reassortment amongst rotavirus isolates..- The neutralisation of the indirect immunofluorescence test and the immuno-diffusion test: two detection methods for neonatal calf diarrhoea viruses. A comparative study with ELISA and electron microscopy..- Comparison of diagnostic procedures for bovine rotavirus..- The diagnosis of Coronavirus-like agent (CVLA) diarrhea in suckling pigs..- Comparison of diagnostic techniques for research in neonatal diarrhea..- Laboratory manuals.- Agricultural Research Council’s Institute for Research on Animal Diseases, Compton, Nr. Newbury, Berks., U.K.:.- - Electron microscopy for the detection of bovine and porcine rotaviruses, bovine enteric Coronavirus, calicivirus-like agents and astroviruses in faeces.- - Detection of porcine and bovine rotaviruses in faeces by infectivity assay in cell culture.- - Detection of bovine astroviruses in faeces by infectivity assay in cell culture.- - Detection of bovine enteric coronaviruses in faeces by inoculation of trachea organ cultures.- - Rotavirus neutralisation test in cell culture by a fluorescent focus reduction test.- Station de Recherches de Virologie et d’Immunologie INRA, 78850-Thiverval-Grignon, France:.- 1. ELISA for the detection and quantitation of rotavirus in faeces.- 2. ELISA for the detection and titration of bovine anti rotavirus Ig in sera.- 3. ELISA for the detection of pig anti rotavirus IgG, IgA and IgM in faeces.- 4. ELISA for the detection of rotavirus/antibody complexes in porcine faeces.- 5. Neutralizing antibody plaque assay for bovine rotavirus.- 6. Detection of bovine enteric Coronavirus (BECV) in HRT 18 cells.- Central Veterinary Institute, Virology Department, 8221 RA Lelystad, the Netherlands:.- - ELISA for the detection of bovine rotavirus antigens in faeces.- - Haemadsorption-elution-haemagglutination assay (HEHA) for the detection of bovine Coronavirus antigens in faeces.- Central Veterinary Institute, the Netherlands and Institute of Medical Microbiology, University of Copenhagen, Department of Clinical Virology, EK-2100 Copenhagen, Denmark:.- - Immunoelectroosmophoresis (IEOP) for the detection of bovine rotavirus antigen in faeces.- Institute of Medical Microbiology, Copenhagen, Denmark:.- - Enzyme linked immunosorbent assay (ELISA) for the detection of rotavirus antigens in faeces.- National Institute for Veterinary Research, Groeselenberg 99, 1180 Brussels, Belgium:.- - Immunodiffusion test for the detection of rotavirus antigen in faecal material and gut homogenates.- - Neutralisation of the indirect immunofluorescence test (NIIF) for the detection of rota, corona and BVD-virus in bovine faecal material.- Laboratory for Virology, Faculteit van de Diergeneeskunde, B 9000 Gent, Belgium:.- - IF-test in the diagnosis of viral gastroenteritis in pigs.- Enteropathogenic E. Coli.- Escherichia coli associated with neonatal diarrhoea in piglets and calves..- This paper includes seven manuals: 1. Preparation of cultures, LT and ST for in vivo or in vitro assay.- 2. Assay of ST in the suckling mouse test.- 3. The ligated gut test (LGT) in pigs.- 4. Assay of LT in the Vero cell test.- 5. Production of the adhesins K88, K99 and P.- 6. A. Preparation of E. coli O, K and H antisera; a general outline.- B. Principles of serotyping E. coli.- C. Preparation of antisera for the detection of bovine and porcine enteropathogenic E. coli strains.- 7. A. Bacteriological techniques recommended for the detection of enterotoxigenic E. coli strains in calves in diagnostic field-laboratories.- B. Bacteriological techniques recommended for the detection of enterotoxigenic E. coli strains in neonatal and post-weaning E. coli enterotoxin induced diarrhoea (and oedema-disease) in piglets.- Adhesion of Escherichia coli pathogenic in pigs, calves and lambs to intestinal epithelial cell brush-borders..- Manual of the brush-border test.- Preliminary experiences with an enzyme-linked immunosorbent assay (ELISA) for the detection of the K88 antigen of E. coli in porcine faeces..- Manual of the K88 ELISA.- Hydrophobic interaction chromatography (laboratory manual)..- Miscellaneous.- Mixed diarrhoeal infection in calves: the relative importance of 2 interacting enteropathogens..- Piglet enteritis; field and laboratory evaluation of enteropathogens associated with 3 disease entities..- Cryptosporidiosis in calves: significance within the enteritis syndrome and diagnosis of infection..- List of Participants.