Riboswitches

Predicting Riboswitch Regulation on a Genomic Scale.- Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications.- Application of Fluorescent Measurements for Characterization of Riboswitch–Ligand Interactions.- Transcriptional Approaches to Riboswitch Studies.- Kinetics of Riboswitch Regulation Studied By In Vitro Transcription.- Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches.- Methods for Analysis of Ligand-Induced RNA Conformational Changes.- Monitoring RNA–Ligand Interactions Using Isothermal Titration Calorimetry.- Preparation and Crystallization of Riboswitch–Ligand Complexes.- Crystallization of the glmS Ribozyme-Riboswitch.- Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering.- Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches.- Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2’-Hydroxyl Acylation and Primer Extension.- Identification of Metabolite–Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression.- RNA-Dependent RNA Switches in Bacteria.- Probing mRNA Structure and sRNA–mRNA Interactions in Bacteria Using Enzymes and Lead(II).- Structural Probing of RNA Thermosensors.- Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria.- Isolation and Characterization of the Heat Shock RNA 1.- Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo.- In Vitro Selection of Conformational Probes for Riboswitches.- A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo.-High-Throughput Screens to Discover Synthetic Riboswitches.- A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage.- In Vitro Selection of glmS Ribozymes.