Introduction to CRISPR-Cas9 Techniques

Dr. Michael Wolyniak studied genetics and biochemistry at Cornell University in Ithaca, New York, USA, and received his Ph.D. in 2004. He did postdoctoral work at the Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA in microbiology and immunology from 2004-2007 and in cell biology and genetics from 2007-2009.  In the latter years of his time at Dartmouth, Wolyniak was a Howard Hughes Medical Institute postdoctoral teaching fellow, giving him the opportunity to teach coursework in cancer biology and first-year composition while conducting his research. In 2009, Wolyniak accepted an appointment to the Biology Department at Hampden-Sydney College in Farmville, Virginia, USA.  He is currently the McGavacks Professor of Biology and Director of Undergraduate Research at Hampden-Sydney.  He has received several National Science Foundation grants in support of projects associated with undergraduate research in the STEM classroom, including the "CRISPR in the Classroom" project that is the inspiration for this volume. Wolyniak has also served on committees for the American Society for Cell Biology, the American Society for Biochemistry and Molecular Biology, the Council on Undergraduate Research, and the Virginia Academy of Science.

Dr. Donna Pattison earned her PhD in Biochemistry and Cell Biology at Rice University and postdoctoral work at Baylor College of Medicine in the Department of Molecular Virology and Microbiology.  Prior to her graduate work, she obtained an undergraduate degree in Curriculum & Instruction from the University of Maryland, College Park.  She taught middle school science for 3 years, serving as the 8th grade faculty team leader and as a district curriculum writer. Dr. Pattison has taught at the University of Houston in the Department of Biology and Biochemistry since 2006, currently serving as an Instructional Professor, Coordinator of the Undergraduate Biochemistry Teaching Laboratory courses, and Assistant Dean for Student Success in the College of Natural Sciences and Mathematics.  She is the current primary investigator for the CRISPR in the Classroom Network, a Research Coordination Network-Undergraduate Biology Education funded through the National Science Foundation. Dr. Pattison's research is in the area of development and assessment of student success programs to improve student learning outcomes and improve undergraduate retention and graduation rates. Dr. Pattison has been an active member of the Association for Biology Laboratory Education, since 2009, serving as Co-Vice President (2015-2017) and as a Member-at-Large on the Board of Directors from 2019-2024. 

Dr. Jay Pieczynski is an Associate Professor and Chair of Biology at Rollins College in Winter Park, FL.  He obtained his Ph.D. in Biological Chemistry from the University of Michigan in 2010 in the laboratory of Dr. Benjamin Margolis, studying the mechanisms of epithelial cell polarity.  From 2010-2012, he was a post-doctoral trainee in the lab of Dr. Bradley Yoder at the University of Alabama-Birmingham where he began using C. elegans as an in vivo model to study neuronal cell development and cilia biology.  Prior to joining the faculty at Rollins, he held teaching positions at the University of Michigan and Vassar College.  At Rollins, he teaches General Biology for both majors and non-majors and Molecular Biology.  Dr. Pieczynski's laboratory research uses the power of C. elegans biology to understand how kinesin motor proteins and microtubules function in vivo.  In addition, he also performs molecular genetics field research, developing and deploying CRISPR-based diagnostic tools as they apply to conservation biology, specifically the expansion of hybrid populations and loss of species diversity due to global climate change and human impacts.  In the classroom, his pedagogy rese

Part I: Foundations of CRISPR in the Classroom .- Chapter 1: Teaching the CRISPR Revolution. Kevin Davies PhD, Executive Editor, The CRISPR Journal, Mary Ann Liebert Inc. 140 Huguenot St, New Rochelle, New York, USA. .- Chapter 2: Expansions on CRISPR-Cas9 technology: Innovations for the future. Dr. Anil Challa, University of Alabama at Birmingham, Alabama, USA .- Chapter 3: CRISPR for the high school classroom. Dr. David Wollert, Chattanooga State Community College, Chattanooga, USA .- Chapter 4: CRISPR for Introductory-Level Undergraduate Courses. Dr. Michael Wolyniak, Hampden-Sydney College, Virginia, USA .- Chapter 5: CRISPR for course-based undergraduate research experiences. Dr. Jay N. Pieczynski, Department of Biology, Rollins College, Winter Park, USA. Dr. Maria S. Santisteban, Biology Department, University of North Carolina Pembroke, Pembroke, USA .- Part II: Model Systems: Do's and Don'ts for Each System .- Chapter 6: CRISPR in Plants. Drs. Nicholas J. Ruppel, Department of Biology, Randolph-Macon College, Virginia, USA. Dawn Carter, Rochester Institute of Technology, Thomas H. Gosnell School of Life Sciences, New York, USA .- Chapter 7: CRISPR in Zebrafish. Dr. Anil Challa, University of Alabama at Birmingham, Alabama, USA .- Chapter 8: CRISPR in Drosophila. Kumar Vishal, Department of Biological Sciences, San José State University, San José, California, USA. Jeffrey L. Van Zant, Department of Biology, Hillsdale College, Hillsdale, Michigan, USA. Richard M. Cripps, Department of Biology, San Diego State University, San Diego, California, USA .- Chapter 9: CRISPR in Yeast. Dr. Randi Ulbricht, Missouri State University, Missouri, USA .- Chapter 10: CRISPR in Butterflies. Dr. Hooi Lynn Kee, Stetson University, DeLand, Florida, USA .- Part III: Additional Applications of CRISPR in the Classroom .- Chapter 11: Navigating Computational Resources for the CRISPR Classroom. Dr Linnea Anderson, North Carolina State University, North Carolina, USA. Dr Carlos Goller, North Carolina State University, North Carolina, USA. Dr Leigh Ann Samsa, University of North Carolina-Chapel Hill, North Carolina, USA. Dr Arnab Senguta, Georgia College and State University, Giorgia, USA .- Chapter 12: Scaffolding CRISPR Lessons to Accommodate Learning Levels and Resource Availability. Dr. Donna Pattison, University of Houston, Texas, USA.