Phage Engineering and Analysis

Protocols for Building and Producing High Diversity Peptide Phage Display Libraries.- Generation of a Naïve Human scFv Phage Display Library and Panning Selection.- Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance.- Combining a Base Deaminase Mutator with Phage-Assisted Evolution.- Understanding the Structural Requirements of Peptide-Protein Interaction and Applications for Peptidomimetic Development.- Contracting the Host Range of Bacteriophage T7 Using a Continuous Evolution System.- Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip.- Targeted Genome Editing of Virulent Pseudomonas Phages using CRISPR-Cas3.- Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods.- Liquid Glycan Array.- Electron Microscopy Methods for Phage-Based Study.- Visualization of Engineered M13 Phages Bound to Bacterial Targets by Transmission Electron Microscopy.- Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination using Cryo-Electron Microscopy.- Obtaining Detailed Phage Transcriptomes using ONT-Cappable-Seq.- Multi-Disciplinary Methods for Screening Toxic Proteins from Phages and Their Potential Molecular Targets.- CRISPRpi: Inducing and Curing Prophage using CRISPR-Interference.- Isolation of Bacteriophages on Actinobacteria Hosts.