Characterization of Nanoparticles Intended for Drug Delivery

Advancements in Nanoparticle Characterization.- Nanoparticle Size Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow Fractionation.- Assessment of Protein Binding Using Asymmetric-Flow Field-Flow Fractionation Combined with Multi-Angle Light Scattering and Dynamic Light Scattering.- Quantitation of Active Pharmaceutical Ingredients in Polymeric Drug Products Using Elemental Analysis.- Particle Size and Concentration Measurement Using the Spectradyne nCS1 Instrument.- Measuring Size and Number Concentration of Metallic Nanoparticles Using spICP-MS.- Ion Quantitation in Liposomal Products with RP-HPLC and Charged Aerosol Detection.- A Static Headspace Gas Chromatography Method for Quantitation of Residual Solvents in Nanoformulations.- Current Considerations and Practical Solutions for Overcoming Nanoparticle Interference with LAL Assays and Minimizing Endotoxin Contamination.- Detection of Beta-Glucan Contamination inNanoparticle Formulations.- Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytes.- Analysis of Nanoparticles’ Potential to Induce Autoimmunity.- Analysis of Nanoparticles’ Effects on Drug-Induced Psoriasis.- Detection of Nanoparticle-Mediated Total Oxidative Stress in T-Cells Using CM-H2DCFDA Dye.- Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T-Cells Using MitoSOX Red Dye.- Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T-Cells Using JC-1 Dye.- Detection of Antigen Presentation by Murine Bone Marrow-Derived Dendritic Cells After Treatment with Nanoparticles.- Antigen-Specific Stimulation of CD8+ T-Cells by Murine Bone Marrow-Derived Dendritic Cells After Treatment with Nanoparticles.- Detection of Pre-Existing Antibodies to Polyethylene Glycol and PEGylated Liposomes in Human Serum.- In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed-Type Hypersensitivity Reactions.- Analysis of Nanoparticle Adjuvant Properties.- Methods for Analysis of Nanoparticle Immunosuppressive Properties.- Evaluation of the Acute Anaphylactoid Reactogenicity of Nanoparticle-Containing Medicines and Vaccines Using the Porcine CARPA Model.- Immunophenotyping, Part I: Instrument Calibration and Reagent Qualification for immunophenotyping Analysis of Human Peripheral Blood Mononuclear Cell Cultures.- Immunophenotyping, Part II: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells.- Understanding the Role of Scavenger Receptor A1 in Nanoparticle Uptake by Murine Macrophages.- Assessment of Temperature-Dependent Drug Release of Solubilizing Nanoformulations Using the SITUA.- Evaluation of Nanomedicine Tissue Distribution and Stability by Alexa Fluor 488 and PEG Immunohistochemistry.