A Practical Guide to Molecular Cloning

Laboratory Equipment Needed for Molecular Cloning; A Few Words about Safety; Basic Methods in Molecular Biology; Useful Modifying Enzymes for Molecular Cloning; Restriction Endonucleases; Vectors for Molecular Cloning; Purification and Characterization of Vector and Passenger DNA; Digestion of DNA with Restriction Endonucleases; Separation of DNA Fragments by Electrophoresis; Ordering the Restriction DNA Fragments in Physical Maps; Purification of DNA Fragments; Modification of DNA Fragments with Cohesive Termini; Ligation; Propagation of Recombinant DNA Molecules; Characterization of Recombinant Clones; Preparation of Genomic Libraries; Purification and Characterization of RNA Species; Cloning of cDNA Species; Sequencing of DNA; RNA Sequencing; Use of Computerized Programs in the Treatment of DNA Sequence Information; Localized Mutagenesis; Methods for Studying DNA-Protein Interactions; Expression of Cloned DNA Sequences In Vitro or In Procaryotic and Eucaryotic Cells; Epilogue: If You Want AVA I to Meet BAM Hi; Appendixes.