Food Allergens -

Food Allergens

Methods and Protocols

Beatriz Cabanillas (Herausgeber)

Buch | Hardcover
377 Seiten
2023 | 1st ed. 2024
Springer-Verlag New York Inc.
978-1-0716-3452-3 (ISBN)
235,39 inkl. MwSt
This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to allergen structure description, cellular responses, and tolerance induction, through a variety of techniques and animal models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Food Allergens: Methods and Protocols serves as an ideal reference for scientists at all stages involved in the study of food allergy and allergenic components.

lt;p>1. Food Allergens of Plant and Animal Origin: Classification, Characteristics, and Properties

            Beatriz Moya, Maria Carmen Dieguez, Jesus F. Crespo, and Beatriz Cabanillas

 

2. Purification of Food Allergens from Their Natural Sources: Chromatographic Methods

            Sahil Gupta and Changqi Liu

 

3. Recombinant Production of Food Allergens in Yeast Pichia pastoris

            Surapon Piboonpocanun

 

4. Yeast Surface Display Methodology for the Characterization of Food Allergens In Situ

            Milica Popovic and Marija Gavrovic-Jankulovic

 

5. Characterization of Linear IgE-Binding Epitopes in Food Allergens

            Changqi Liu, Sahil Gupta, and Jing Zhao

 

6. Characterization of T-Cell Epitopes in Food Allergens by Bioinformatic Tools

            Shudong He and Fanlin Zhou

 

7. Phage Immunoprecipitation Sequencing (PhIP-Seq) for Analyzing Antibody Epitope Repertoires against Food Antigens

            Ioanna Filimonova, Gabriel Innocenti, and Thomas Vogl

 

8. 1D-, 2D-Gel Electrophoresis, Immunoblotting, and Enzyme-Linked Immunosorbent Assay (ELISA) for the Study of Food Allergens

            Joana Costa, Caterina Villa, and Isabel Mafra

 

9. Preparation of Blinded Food Matrixes for Clinical Oral Challenges

            Julie A. Nordlee, Joe L. Baumert, and Steve L. Taylor

 

10. Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy

            Ricarda Zeindl, Jana Unterhauser, Manuel Röck, Reiner Eidelpes, Sebastian Führer, and Martin Tollinger

 

11. Co-Culture of Human Dendritic and T Cells for the Study of Specific T Cell-Mediated Responses against Food Allergens

            Mónica Martínez-Blanco, David Menchén-Martínez, Carmen Cámara, Rosina López-Fandiño, M. Cecilia Berin, and Daniel Lozano-Ojalvo

 

12. Evaluation of the Suppressive Capacity of Regulatory T Cells in Food Allergy Research

            David Menchén-Martínez, Mónica Martínez-Blanco, Daniel Lozano-Ojalvo, and M. Cecilia Berin

 

13. Mass Cytometry in Food Allergy Research

            Nathalie Acevedo and Kevin Llinás-Caballero

 

14. Indirect Basophil Activation Test for Peanut Allergy Diagnosis Using Human Donor Basophils

            Janneke Ruinemans-Koerts and Yvonne Schmidt-Hieltjes

 

15. Standardization of Food Allergen Measurements Using Multiplex Array Technology

            Kristina Reid Black, Stephanie C. Filep, Bryan R.E. Smith, and Martin D. Chapman

 

16. Biosensors for the Detection of Food Allergens

            Alina Vasilescu, Cristina Polonschii, and Jean-Louis Marty

 

17. Standardization of a Mass Spectrometry-Based Workflow for Food Allergen Quantification

            Rosa Pilolli, Elisabetta De Angelis, Antonella Lamonaca, and Linda Monaci

 

18. Identifying Similar Allergens and Potentially Cross-Reacting Areas Using Structural Database of Allergenic Proteins (SDAP) Tools and D-Graph

            Catherine H. Schein

 

19. Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA)

            Changqi Liu, Mengna Su, Valerie D. Zaffran, and Sahil Gupta

 

20. Detection of Bet v 1 Homologous Proteins and Plant Profilins by Indirect ELISA

            Adrian Bartos, Iwona Majak, and Joanna Leszczynska

 

21. A Mouse Model of Shrimp Allergy with Cross-Reactivity to Crab and Lobster

            Johanna M. Smeekens, Janelle R. Kesselring, Ken Bagley, and Michael D. Kulis

 

22. Animal Models in the Study of Food Allergens: Long-Term Maintenance of Allergic Reactivity in Mouse Models of Food Allergy

            Jessica J. O'Konek

 

23. Study of MicroRNAs Expression in Food Allergy

            Emilio Nuñez-Borque, Lucía Palacio-Garcia, Sergio Fernández-Bravo, and Vanesa Esteban

 

24. De Novo Transcriptomic Analyses to Identify and Compare Allergens in Foods

            Shaymaviswanathan Karnaneedi, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, and Andreas L. Lopata

 

25. Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Assay in Food Allergy Research

            Fahd Alhamdan

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology
Zusatzinfo 57 Illustrations, color; 16 Illustrations, black and white; XII, 377 p. 73 illus., 57 illus. in color. With online files/update.
Verlagsort New York, NY
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Dermatologie
Studium Querschnittsbereiche Infektiologie / Immunologie
Technik Lebensmitteltechnologie
Schlagworte Allergenic components • Animal Models • Bioinformatics • Food allergy mechanisms • therapeutic targets
ISBN-10 1-0716-3452-6 / 1071634526
ISBN-13 978-1-0716-3452-3 / 9781071634523
Zustand Neuware
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