R-Loops
Springer-Verlag New York Inc.
978-1-0716-2479-1 (ISBN)
Authoritative and practical, R-Loops: Methods and Protocols serves as an ideal resource for those working on R-loop homeostasis but also to scientists studying such areas of molecular and cell biology as genome integrity, DNA replication and repair, chromatin remodeling, transcription, RNA processing, modification and export, as well as for researchers elucidating the molecular mechanisms of cancer and genetic diseases.
lt;p>1. Direct R-Loop Visualization on Genomic DNA by Native Automated Electron Microscopy
Henriette Stoy, Joel Luethi, Fabienne Roessler, Johannes Riemann, Andres Kaech, and Massimo Lopes
2. Detection of R-Loop Structures by Immunofluorescence Using the S9.6 Monoclonal Antibody
Konstantina Skourti-Stathaki
3. R-Loop Detection in Bacteria
Émilie Vlachos-Breton and Marc Drolet
4. Detection of R-Loops by In Vivo and In Vitro Cytosine Deamination in Saccharomyces cerevisiae
Juan C. Cañas, Andrés Aguilera, and Belén Gómez-González
5. Identification of RNA-DNA Hybrids Associated with R-Loops at the IgH Switch Sequence in Activated B Cells
Junghyun Lim, Brice Laffleur, Uttiya Basu, and Kefei Yu
6. Locus-Specific Analysis of Replication Dynamics and Detection of DNA:RNA Hybrids by Immuno Electron Microscopy
Henriette Stoy, Kevin Lang, Houra Merrikh, and Massimo Lopes
7. RNase H1, the Gold Standard for R-Loop Detection
Susana M. Cerritelli, Kiran Sakhuja, and Robert J. Crouch
8. RNase H1 Hybrid-Binding Domain-Based Tools for Cellular Biology Studies of DNA-RNA Hybrids in Mammalian Cells
Sónia Silva, Cristina Guillén-Mendoza, and Andrés Aguilera
9. Detection and Quantification of RNA Modifications on RNA/DNA Hybrids Using SID-UPLC-MS/MS
Marta Starczak, Abdulkadir Abakir, Alexey Ruzov, and Daniel Gackowski
10. DNA:RNA Hybrids at Telomeres in Budding Yeast
Carolin B. Wagner and Brian Luke
11. Detection of TERRA R-Loops at Human Telomeres
Galina Glousker, Rita Valador Fernandes, Marianna Feretzaki, and Joachim Lingner
12. R-Loops and Mitochondrial DNA Metabolism
Ian J. Holt
13. Highly-Purified Top1-Bound DNA Fragments
Jessica Marinello and Giovanni Capranico
14. R-Loop Immunoprecipitation: A Method to Detect R-Loop Interacting Factors In Vivo
Chiara Beghè and Natalia Gromak
15. Studies on Protein-RNA:DNA Hybrid Interactions by Microscale Thermophoresis (MST)
Miaomiao Li, Arne Klungland, and Bjørn Dalhus
16. Studying R-Loop Recognizing Proteins Using Single-Molecule DNA Curtain Technique and Electrophoretic Mobility Shift Assay
Na Young Cheon and Ja Yil Lee
17. Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids
Haibo Yang and Li Lan
18. In Vitro Binding of GADD45A to RNA:DNA Hybrids
Khelifa Arab and Christof Niehrs
19. Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage
Adele Alagia, Ruth F. Ketley, and Monika Gullerova
20. Biochemical Analysis of RNA-DNA Hybrid and R-Loop Unwinding via Motor Proteins
Arijit Dutta, Youngho Kwon, and Patrick Sung
21. Topology of RNA:DNA Hybrids and R-Loops in Yeast
Yathish Jagadheesh Achar and Marco Foiani
22. Detecting and Mapping N6-Methyladenosine on RNA/DNA Hybrids
Abdulkadir Abakir, Fahad Alenezi, and Alexey Ruzov
23. Genome-Wide Native R-Loop Profiling by R-Loop Cleavage Under Targets and Tagmentation (R-Loop CUT&Tag)
Honghong Wang, Conghui Li, and Kaiwei Liang
24. Mapping R-Loops Using Catalytically Inactive RNaseH1 (R-ChIP)
Xuan Zhang, Yajing Hao, and Xiang-Dong Fu
25. Targeted Nuclease Approaches for Mapping Native R-Loops
Phillip Wulfridge, Qingqing Yan, and Kavitha Sarma
26. Quantitative DNA-RNA Immunoprecipitation-Sequencing with Spike-Ins
Magdalena P. Crossley and Karlene A. Cimprich
27. DNA:RNA Immunoprecipitation from S. pombe Cells for qPCR and Genome-Wide Sequencing
Laetitia Vachez, Camille Teste, and Vincent Vanoosthuyse
28. Genome-Wide Analysis of DNA-RNA Hybrids in Yeast by DRIPc-Seq and DRIP-Seq
María García-Rubio, María E. Soler-Oliva, and Andrés Aguilera
29. Quantitative, Convenient, and Efficient Genome-Wide R-Loop Profiling by ssDRIP-Seq in Multiple Organisms
Wei Xu, Kuan Li, Qin Li, Shuai Li, Jincong Zhou, and Qianwen Sun
Erscheinungsdatum | 21.06.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 1 Illustrations, black and white; XIV, 470 p. 1 illus. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Technik ► Maschinenbau | |
Schlagworte | DNA-RNA hybrids • electron microscopy • Genetic diseases • Genome Dynamics • Genome-wide mapping • Genomic stress and instability • Topology |
ISBN-10 | 1-0716-2479-2 / 1071624792 |
ISBN-13 | 978-1-0716-2479-1 / 9781071624791 |
Zustand | Neuware |
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