PCR Sequencing Protocols

Preparation and Analysis of DNA Sequencing Gels

Purification of PCR Products from Agarose Gels for Direct Sequencing

Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing

Direct Sequencing of Double-Stranded PCR Products with the Sequencase Kit and [a-35S] dATP

Direct Sequencing by Thermal Asymmetric PCR

Rapid Sequencing of cDNA Clones: Direct Sequencing Using Sequential Linear/Asymmetric PCR

Direct Sequencing of PCR Products Using Chemiluminescent Detection

Direct DNA Sequencing of PCR Products Using Magnetic Beads

Affinity Capture and Solid-Phase Sequencing Biotinylated PCR Products

Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing.

Nonradioactive PCR Sequencing Using Digoxygenin

Silver SequencingTM: Nonradioactive Cycle Sequencing of dsDNA

Direct Sequencing of PCR Products with DNA-Binding Proteins

PCR Sequencing with the Aid of Detergents

Direct Sequencing with Highly Degenerate and Inosine-Containing Primers

Determination of Unknown Genomic Sequences Without Cloning

DNA Sequencing by the Chemical Method

Direct PCR Sequencing with Denaturants (Formamide)

Efficient PCR Production of Single-Stranded DNA Sequencing Templates

Preparation and Direct Automated Cycle Sequencing of PCR Products

Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA

Cloning PCR Products for Sequencing in M13 Vectors

Sequencing PCR Products Cloned into M13 Vectors

Genome Amplification with Transcript Sequencing (GAWTS)

DNA Rescue by Vectorette Method

Sequencing (dAidT) of Cloned Mixed PCR Products from Microbial Populations

Index