Targeted Protein Degradation

 Protein Ligand Interactions Using Surface Plasmon Resonance.- High-Throughput Detection of Ligand-Protein Binding using a SplitLuc Cellular Thermal Shift Assay.- Proteins and their Interacting Partners: An Introduction to Protein-Ligand Binding Site Prediction Methods with a Focus on FunFOLD3.- Evaluating ligands for ubiquitin ligases using affinity beads.- Mechanistic and Structural Features of PROTAC Ternary Complexes.- MST and TRIC Technology to Reliably Study PROTAC Binary and Ternary Binding In Drug Development.- An in vitro Pull-down Assay of the E3 ligase:PROTAC:Substrate Ternary Complex to Identify Effective PROTACs.- Kinetic detection of E3: PROTAC: Target Ternary Complexes using NanoBRET technology in Live Cells.- Inducing Ubiquitylation and Protein Degradation as a Drug Development Strategy.- Methodological Versatility of Tandem Ubiquitin Binding Entities (TUBEs) for Understanding Poly-ubiquitination and its Various Chains.- Global Mass Spectrometry-Based Analysis of Protein Ubiquitination Using K-e-GG Remnant Antibody Enrichment.- Determination of Proteasomal Unfolding Ability.- Methods for Quantitative Assessment of Protein Degradation.- A High Throughput method to prioritize PROTAC intracellular target engagement and cell permeability using NanoBRET.-  Profiling CELMoD Mediated Degradation of Cereblon Neosubstrates.- Global Proteome Profiling to Assess Changes in Protein Abundance using Isobaric Labeling and Liquid Chromatography Tandem Mass Spectrometry.- PHOTACs Enable Optical Control of Protein Degradation.- Protocols for synthesis of SNIPERs and the methods to evaluate the anti-cancer affects.