Molecular Genetics of Yeast
Oxford University Press (Verlag)
978-0-19-963430-9 (ISBN)
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The yeast Saccharomyces cerevisiae is used extensively in academic research and industry as a model eukaryote. It is easy to manipulate, is very flexible, and has a great many parallels with higher organisms. This makes it ideal for studies in molecular biology, genetics and biotechnology. This text describes current experimental procedures for working with the organism.
1: Procedures for isolating yeast DNA for different purposes. 2: Construction of cloning and expression vectors. 3: Cosmid cloning of yeast DNA. 4: The construction and use of cDNA libraries for genetic selections. 5: Pulsed field gel electrophoresis. 6: Plasmid shuffling and mutant isolation. 7: Ty insertional mutagenesis. 8: High efficiency transformation with lithium acetate. 9: Measurement of transcription. 10: Measurement of mRNA stability. 11: Production of foreign proteins at high level. 12: Cell-free translation of natural and synthetic mRNAs. 13: Virus-like particles: Ty retrotransposons. 14: The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly. 15: Immuno-electron microscopy. 16: Industrial Saccharomyces yeasts
| Erscheint lt. Verlag | 1.6.1994 |
|---|---|
| Zusatzinfo | halftones, line drawings, tables, bibliography |
| Verlagsort | Oxford |
| Sprache | englisch |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Naturwissenschaften ► Biologie ► Mykologie | |
| Technik ► Umwelttechnik / Biotechnologie | |
| ISBN-10 | 0-19-963430-0 / 0199634300 |
| ISBN-13 | 978-0-19-963430-9 / 9780199634309 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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