CRISPR Guide RNA Design

Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experiments.- Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer.- Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells.- Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNA.- ‘Split-and-Click’ sgRNA.- Chimeric DNA-RNA Guide RNA Designs.- Harnessing tRNA for Processing Ability and Promoter Activity.- Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA–ncRNA Expression Constructs.- Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs.- Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a.- Methods for Measuring CRISPR/Cas9 DNA Cleavage in Cells.- In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR/Cas9 Systems.- Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq.- Detection of CRISPR/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral Vector.- Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method.