Corneal Regeneration

Isolation and Culture of Corneal Stromal Stem Cells.- In vitro Expansion of Corneal Endothelial Cells for Transplantation.- Primary Culture of Cornea-Limbal Epithelial Cells In Vitro.- Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant.- A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture.- Gene Editing for Corneal Stromal Regeneration.- Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery.-The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea.- Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding.- Preparation of Dried Amniotic Membrane for Corneal Repair.- Fabrication of Corneal Extracellular Matrix Derived Hydrogels.- Synthesis and Application of Collagens for Assembling a Corneal Implant.- Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts.- Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration.- The use of Animal Models to Assess Engineered Corneal Tissue.- X-ray Diffraction Imaging of Corneal Ultrastructure.