Plant and Food Carotenoids

Pathways for Carotenoid Biosynthesis, Degradation, and Storage.- A Simple in vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-chain Prenyltransferases.- Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase in vitro.- Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO.- Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases.- Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases.- Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters.- Applications of Visible Spectroscopy and Colour Measurements in the Assessments of Carotenoid Levels in Foods.- A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids.- Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management.- cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues.- Determination of Plant Volatile Apocarotenoids.- A Method for Extraction and LC-MS Based Identification of Carotenoid-Derived Dialdehydes in Plants.- Fractionation of Tomato Fruit Chromoplasts.- Quantification of Strigolactones.- Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS.- Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy.- Visualization of Carotenoid-storage Structures in Fruits by Transmission Electron Microscopy.-  Light Microscopy and Raman Imaging of Carotenoids in Plant Cells in situ and in Released Carotene Crystals.- Carotenoid Isotopologue Profiling in 13C-labeled Leaf Extracts by LC-MS and LC-FTICR-MS.- Quantification of Carotenoid Pathway Flux in Green and Non-green Systems.- Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis.- Use of Potyvirus Vectors to Produce Carotenoids in Plants.- Agrobacterium tumefaciens Mediated Stable Transformation of Daucus carota.- CRISPR-Cas-Mediated Gene Knockout in Tomato.- Modulation of Activity of a Carotenoid Pathway Through the use of the TET-on Regulatory System: Application in the Fungus Fusarium fujikuroi.- Intervention Studies in Humans.- In vitro Digestion for Control and Monitoring of Food Effects In Relation To Micellarization Index of Carotenoids.- Evaluation of Carotenoids Protection against Oxidative Stress in the Animal Model Caenorhabditis elegans.- Mouse Models to Study Antiobesogenic Effects of Carotenoids.