Stem Cells and Aging
Humana Press Inc. (Verlag)
978-1-4939-9715-2 (ISBN)
Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study.
Assessing Muscle Stem Cell Clonal Complexity during Aging.- Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.- Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle.- Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue.- Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures.- Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells.- Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis.- Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells.- Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis.- 3D-Embedded Cell Cultures to Study Tendon Biology.- Targeted, Amplicon-Based,Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis.- Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants.- Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice.- Infrared Spectroscopy and Imagıng in Stem Cells and Agıng Research.- Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres.- Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry.- Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry.- Methods for Detection of Autophagy in Mammalian Cells.- Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells.- Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer.- Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD).- Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury.- 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol.- Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments.- CRISPR Base Editing in Induced Pluripotent Stem Cells.
Erscheinungsdatum | 06.09.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 2045 |
Zusatzinfo | 64 Illustrations, color; 3 Illustrations, black and white; XII, 354 p. 67 illus., 64 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | Chromatin immunoprecipitation • Epigenetic regulation • Mesenchymal Stem Cells • Progenitor Cells • Skeletal muscle cells |
ISBN-10 | 1-4939-9715-7 / 1493997157 |
ISBN-13 | 978-1-4939-9715-2 / 9781493997152 |
Zustand | Neuware |
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