Skeletal Development and Repair

 Overview of Skeletal Development.- Overview of Skeletal Repair (Fracture Healing and Its Assessment).- Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research.- Generation of Closed Transverse Fractures in Small Animals.- A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes.- Surgical Induction of Posttraumatic Osteoarthritis in the Mouse.- Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton.- Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development.- Murine Limb Explant Cultures to Assess Cartilage Development.- Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair.- MicroCT for Scanning and Analysis of Mouse Bones.- Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties.- Phenotyping Intact Mouse Bones using Bone CLARITY.- Processing and Sectioning Undecalcified Murine Bone Specimens.- Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020).- Demineralized Murine Skeletal Histology.- Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix.- Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter.- Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue.- Detection of Hypoxic Regions in the Bone Microenvironment.- EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections.- Whole-mount In Situ Hybridization in Murine Tissues.- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo.- Isolation and Culture of Periosteum Derived Progenitor Cells from Mice.- Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts.-Mitochondrial Function and Metabolism of Cultured Skeletal Cells.- Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants.- RANKL-based Osteoclastogenic Assay from Murine Bone Marrow Cells.- Hematopoietic Stem Cell Cultures and Assays.