Microbial Metabolic Engineering

Pathway and Gene Discovery from Natural Hosts and Organisms.- Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity.- Antisense RNA Elements for Downregulating Expression.- Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering.- Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs.- Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae.- Assembly of Complex Pathways using Type IIs Restriction Enzymes.- Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly.- Checks and Balances with Use of the Keio Collection for Phenotype Testing.- Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803.- Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica.- Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization.- Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization.- A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models.- 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms.- A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast.