Nanotechnology for Nucleic Acid Delivery

Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection.- Synthesis of Bioreducible Polycations with Controlled Topologies.- Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery.- Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates.- Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems.- Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids.- Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery.- Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation.- Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol.- Layer-By-Layer Assembled Nanoparticles for siRNA Delivery.- Layer-By-Layer Film Engineering for Sequential Gene Delivery.- Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles.- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films.- Lyophilization of Synthetic Gene Carriers.- Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery.- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles.- Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery.- Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity.- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity.- Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS).- Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining.- Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery.- Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo.- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.- Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation.- Lipopeptide Delivery of siRNA to the Central Nervous System.