Essential Developmental Biology

A Practical Approach

Claudio D. Stern, Peter W. H. Holland (Herausgeber)

Buch | Softcover

368 Seiten

1993
Oxford University Press (Verlag)
978-0-19-963422-4 (ISBN)

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Brings together easy-to-follow protocols and practical instructions for all of the main techniques in classical embryo manipulation, from traditional embryology to cellular and molecular methods. The book includes reprints of all the stage tables in common use for the main laboratory species.

Developmental biology is a newly-developing subject that is attracting much interest because of exciting findings being made using a combination of classical embryo manipulation with more modern techniques. This book brings together for the first time, and in comprehensive, easy-to-follow protocols, practical instructions for all of the main techniques, from traditional embryology to cellular and molecular methods. It includes complete reprints of all the stage
tables in common use for the main laboratory species. It will become an essential addition to laboratory benches of those using any technique as applied to embryos, cells, or tissues. It will also help teachers to design practical classes suitable for undergraduate and graduate courses.

PART I: BASIC TECHNIQUES: OBTAINING AND HANDLING EMBRYOS ; 1. Drosophila embryos ; 2. Nematode embryos ; 3. Embryos and larvae of invertebrate deuterostomes ; 4. Zebrafish embryos ; 5. Xenopus ; 6. Avian embryos ; 7. Post-implantation mammalian embryos ; 8. Simple tips for photomicrography or embryos ; PART II: EXPERIMENTAL EMBRYOLOGY ; 9. Fate maps and cell lineage analysis ; 10. Microinjection into Drosophila embryos ; 11. Cell transplantation in Xenopus ; 12. Transplantation in avian embryos ; 13. Chick limb buds ; 14. Nuclear transplantation ; 15. Laser ablation of cells ; PART III: CELLULAR TECHNIQUES ; 16. Studying cell movements in vivo by time-lapse video microscopy ; 17. Induction assays in Xenopus ; 18. Culture of avian neural crest cells ; 19. ES cells and gene targeting ; 20. Introduction of genes using retroviral vectors ; PART IV: MOLECULAR TECHNIQUES ; 21. Immunocytochemistry of embryonic material ; 22. RNase protection assays ; 23. cDNA library construction ; 24. Analysis of gene expression by RT-PCR ; 25. Cloning genes using PCR ; 26. In situ hybridisation ; Appendices ; I: Stage tables ; a. Westerfield for zebrafish embryos ; b. Nieukoop & Faber for Xenopus laevis ; c. Eyal-Giladi & Kochav for early chick ; d. Hamburger & Hamilton for later chick ; II: Suppliers of specialist items ; Index

Erscheint lt. Verlag	12.8.1993
Reihe/Serie	Practical Approach Series ; 132
Zusatzinfo	halftones, line drawings, tables
Verlagsort	Oxford
Sprache	englisch
Maße	155 x 234 mm
Gewicht	643 g
Themenwelt	Studium ► 1. Studienabschnitt (Vorklinik) ► Histologie / Embryologie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Biologie ► Zoologie
	Technik ► Umwelttechnik / Biotechnologie
ISBN-10	0-19-963422-X / 019963422X
ISBN-13	978-0-19-963422-4 / 9780199634224
Zustand	Neuware