DNA Replication
Humana Press Inc. (Verlag)
978-1-4939-5660-9 (ISBN)
Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.
Reviews.- DNA Replication Initiation.- DNA Replication Fork Proteins.- Random and Site-Specific Replication Termination.- Checkpoint Regulation of DNA Replication.- to Molecular Combing: Genomics, DNA Replication, and Cancer.- Methods for Analysis of Replication of a Single Locus.- Replication Initiation Point Mapping: Approach and Implications.- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis.- Topological Analysis of Plasmid DNA Replication Intermediates Using Two-Dimensional Agarose Gels.- Analysis of Telomeric DNA Replication Using Neutral–Alkaline Two-Dimensional Gel Electrophoresis.- Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork.- Density Transfer as a Method to Analyze the Progression of DNA Replication Forks.- High-Resolution Mapping of Points of Site-Specific Replication Stalling.- DNA Replication in Nucleus-Free Xenopus Egg Extracts.- Genome-Wide Analysis Methods.- ChIP-Chip to Analyze the Binding of Replication Proteins to Chromatin Using Oligonucleotide DNA Microarrays.- Analyzing Origin Activation Patterns by Copy Number Change Experiments.- Detection of Replication Origins Using Comparative Genomics and Recombinational ARS Assay.- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.- Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication After DNA Damage.- Biochemistry and Biophysics Methods.- Isolation of Recombinant DNA Elongation Proteins.- In Vitro Assays for Studying Helicase Activities.- The Use of 2-Aminopurine Fluorescence to Study DNA Polymerase Function.- Single-Molecule Observation of Prokaryotic DNA Replication.- Cell Biology and Genetic Methods.- Visualizationof DNA Replication Sites in Mammalian Nuclei.- Cell-Cycle Synchrony for Analysis of S. pombe DNA Replication.- Measuring DNA Content by Flow Cytometry in Fission Yeast.- Microscopy Techniques to Examine DNA Replication in Fission Yeast.- Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe.- Assays Used to Study the DNA Replication Checkpoint in Fission Yeast.- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast.- The Fast-Halo Assay for the Assessment of DNA Damage at the Single-Cell Level.- Monitoring Homologous Recombination Following Replication Fork Perturbation in the Fission Yeast Schizosaccharomyces pombe.- DNA Fiber Analysis Methods.- Computational Methods to Study Kinetics of DNA Replication.- Use of DNA Combing to Study DNA Replicationin Xenopus and Human Cell-Free Systems.- Electron Microscopy Methods for Studying In Vivo DNA Replication Intermediates.- Determining the Replication Dynamics of Specific Gene Loci by Single-Molecule Analysis of Replicated DNA.- Use of DNA Combing for Studying DNA Replication In Vivo in Yeast and Mammalian Cells.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 521 |
Zusatzinfo | XIII, 701 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 193 x 260 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zoologie | |
Technik ► Umwelttechnik / Biotechnologie | |
ISBN-10 | 1-4939-5660-4 / 1493956604 |
ISBN-13 | 978-1-4939-5660-9 / 9781493956609 |
Zustand | Neuware |
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