Glycosylation Engineering of Biopharmaceuticals

Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells.- Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells.- Glycoengineered Pichia-Based Expression of Monoclonal Antibodies.- N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae.- Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells.- Nano LC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody.- Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry.- Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support.- Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems.- Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC.- Application of Capillary Electrophoresis in Glycoprotein Analysis.- Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology.- Hydrophobic Interaction Chromatography to Analyze Glycoproteins.- Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7.- Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation.- Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes.- Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry.- Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study.- Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Deshydrogenase (LDH) Measurement.- Evaluation of Complement Dependent Cytotoxicity Using ATP Measurement and C1q / C4b Binding.- Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG / FcgR Interaction Using SPR-Based Biosensor Technology.- Mass Spectrometry Protocol for the Absolute Quantification of a Monoclonal Antibody in Serum with Immunopurification.