RNA Detection and Visualization
Humana Press Inc. (Verlag)
978-1-4939-5836-8 (ISBN)
Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.
Single Molecule Imaging of RNA In situ.- FISH and Immunofluorescence Staining in Chlamydomonas.- High Resolution Fluorescent In situ Hybridization in Drosophila.- Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization.- Visualization of mRNA Localization in Xenopus Oocytes.- Visualization of mRNA Expression in the Zebrafish Embryo.- High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments In vitro and In vivo.- Localization of mRNA in Vertebrate Axons Using In situ Hybridization.- Tiny Molecular Beacons for In vivo mRNA Detection.- Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA.- Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells.- Visualization of Induced RNA in Single Bacterial Cells.- Visualizing mRNAs in Fixed and Living Yeast Cells.- In vivo Visualization of RNA Using the U1A-Based Tagged RNA System.- Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based RNA Aptamer Integration Method, and Fluorescence Microscopy.- Imaging mRNAs in Living Mammalian Cells.- Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport during Drosophila Oogenesis.- Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria.- Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation.- Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Affinity Purification Procedure.- Profiling Axonal mRNA Transport.- RNA Purification from Tumor Cell Protrusions Using Porous Polycarbonate Filters.-RNA-Binding Protein Immunopurification-Microarray (RIP-Chip) Analysis to Profile Localized RNAs.- RaPID: An Aptamer-Based mRNA Affinity Purification Technique for the Identification of RNA and Protein Factors Present in Ribonucleoprotein Complexes.- RIP: An mRNA Localization Technique.- The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA-Protein Complexes.- Identifying and Searching for Conserved RNA Localization Signals.- Computational Prediction of RNA Structural Motifs Involved in Post Transcriptional Regulatory Processes.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 714 |
Zusatzinfo | XIV, 497 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Technik ► Maschinenbau | |
ISBN-10 | 1-4939-5836-4 / 1493958364 |
ISBN-13 | 978-1-4939-5836-8 / 9781493958368 |
Zustand | Neuware |
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