Argonaute Proteins

Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe.- Chromatin Immuno-Precipitation in Fission Yeast.- Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies.- Mapping of Ago2-GW182 Functional Interactions.- Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway.- In vitro RISC Cleavage Assay.- Native Gel Analysis for RISC Assembly.- Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes.- Detection of Human Dicer and Argonaute 2 Catalytic Activity.- Imaging the Cellular Dynamics of Drosophila Argonaute Proteins.- Live Cell Imaging of Argonaute Proteins in Mammalian Cells.- Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells.- Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs.- An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro.- Cell-Free microRNA-Mediated Translation Repression in C. elegans.- Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices.- Cloning Argonaute-Associated Small RNAs from C. elegans.- Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs.- Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins.- Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach.